Cell Preparations For Extemporaneous Use, Useful for Healing and Rejuvenation In Vivo

Abstract

The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell combinasons preparations for use in tissue regeneration and bone regeneration and pain reduction.

We claim: 
     
         1 . An isolated cell composition comprising:
 a) plasma;   b) platelets at a concentration of at least 300×10 9  cells/L;   c) white blood cells at a concentration of at least 7.0×10 9  cells/L; and   d) fibrinogen at a concentration of at least 3 mg/L;   
       and wherein the erythrocyte concentration is less than 0.6×10 12  cells/L. 
     
     
         2 . An implantable device for use in tissue regeneration therapy comprising:
 a) a permeable core comprising a cell composition comprising:
 i) plasma; 
 ii) platelets at a concentration of at least 300×10 9  cells/L; 
 iii) white blood cells at a concentration of at least 7.0×10 9  cells/L; 
 iv) fibrinogen at a concentration of at least 3 mg/L; and 
 v) a cell extract where cells are selected from dermal cells, keratinocytes, fibroblasts, melanocytes and Langheran's cells; fat cells; bone marrow cells; muscle cells; osteoblasts; chondrocytes; periosteal membrane cells; corneal cells; umbilical cord cells; Schwann cells, tendon cells and pancreas islet cells, wherein cells are at a concentration of about 10 5  to about 10 6  cells/L and wherein the erythrocyte concentration of said cell composition is less than 0.6×10 12  cells/L; and 
   b) an external jacket surrounding said core, said jacket comprising a biocompatible material or a bioresorbable material.   
     
     
         3 . A device for the preparation of a platelet concentrate from whole blood comprising a separator tube wherein the separator tube is selected from:
 a glass separator tube containing a polyester-based thixotropic gel and a buffered sodium citrate solution at 0.10 M; and   a polyethylene terephthalate separator tube containing a highly thixotropic gel formed by a polymer mixture and an anhydrous sodium citrate at 3.5 mg/mL;   
       characterised in that the device has an inlet for introducing said whole blood, is held in a vacuum intended to aspirate the whole blood sample, is sterile, has a usable vacuum of or about 8 to about 10 mL and is suitable for undergoing centrifugation.