Composition for Use as an Assay

Abstract

A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.

1 . A copolymer of the formula: 
       
         
           
           
               
               
           
         
         wherein: 
         A is O or NR 1  wherein R 1  is H or alkyl of from 1 to 6 carbon atoms; 
         n is 1 to 10; 
         G′ is CHO; CH(OR 8 ) 2  wherein R 8  is alkyl of from 1 to 6 carbon atoms; a member of a specific binding pair; 
         D′ is
 (i) —COOR 10  wherein R 10  is H or alkyl of from 1 to 6 carbon atoms; 
 
       
       
         
           
           
               
               
           
         
         wherein: w is 2-4; or
 (iii) —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ 
 
         wherein: 
         X is O or NR 2  wherein R 2  is H or alkyl of from 1 to 6 carbon atoms; 
         Y is a polyether group of the formula —(CH 2 O) m — wherein m is 2 to 1500, or >N ⊕ (R 3 R 4 ) wherein R 3  and R 4  are independently H or alkyl of from 1 to 6 carbon atoms; 
         p is 0 to 10, being at least 1 when Y is >N ⊕ (R 3 R 4 ); 
         q is 0 or 1; 
         r is 0 to 10, being at least 1 when Y is >N ⊕ (R 3 R 4 ); and 
         Z′ is SO 3   − ; or alkyl of from 1 to 6 carbon atoms; 
         R 5 , R 6  and R 7  are independently H or alkyl of from 1 to 6 carbon atoms; and 
         x and y are independently 1 to about 1000. 
       
     
     
         2 . The copolymer according to  claim 1  wherein:
 A is NR 1  wherein R 1  is H or alkyl of from 1 to 6 carbon atoms; 
 n is 1 to 10; 
 G′ is CHO; CH(OR 8 ) 2  wherein R 8  is alkyl of from 1 to 6 carbon atoms; or a member of a specific binding pair; 
 D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
 X is O; 
 Y is >N ⊕ (R 3 R 4 ) wherein R 3  and R 4  are independently H or alkyl of from 1 to 6 carbon atoms; 
 p is 1 to 10; 
 q is 1; 
 r is 1 to 10; 
 Z′ is SO 3   − ; 
 
 R 5 , R 6  and R 7  are independently H or alkyl of from 1 to 6 carbon atoms; and 
 x and y are independently 1 to 1000. 
 
     
     
         3 . The copolymer according to  claim 2  wherein:
 A is NH; 
 n is 1; 
 G′ is CHO; CH(OR 8 ) 2  wherein R 8  is methyl; or a member of a specific binding pair; 
 D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
 X is O; 
 Y is >N ⊕ (R 3 R 4 ) wherein R 3  and R 4  are both methyl; 
 p is 2; 
 q is 1; 
 r is 3; 
 Z′ is SO 3   − ; 
 
 R 5  and R 6  are independently H or methyl and R 7  is H; and 
 x and y are independently 1 to 1000. 
 
     
     
         4 . The copolymer according to  claim 1  wherein:
 A is NR 1  wherein R 1  is H or alkyl of from 1 to 6 carbon atoms; 
 n is 1 to 10; 
 G′ is CHO; CH(OR 8 ) 2  wherein R 8  is alkyl of from 1 to 6 carbon atoms; or a member of a specific binding pair; 
 D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
 X is O; 
 Y is a polyether group of the formula —(CH 2 O) m — wherein m is 2 to 1500; 
 p is 0; 
 q is 1; 
 r is 0; 
 Z′ is methyl; 
 
 R 5 , R 6  and R 7  are independently H or alkyl of from 1 to 6 carbon atoms; and 
 x and y are independently 1 to 1000. 
 
     
     
         5 . The copolymer according to  claim 4  wherein:
 A is NH; 
 n is 1; 
 G′ is CHO; CH(OR 8 ) 2  wherein R 8  is methyl; or a member of a specific binding pair; 
 D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
 X is O; 
 Y is a polyether group of the formula —(CH 2 O) m — wherein m is 1100 or 300; 
 p is 0; 
 q is 1; 
 r is 0; 
 Z′ is methyl; 
 
 R 5  and R 6  are independently H or methyl and R 7  is H; and 
 x and y are independently 1 to 1000.