CULTURING CIRCULAR ssDNA VIRUSES FOR THE PRODUCTION OF VACCINES

Abstract

The present invention relates to the use of interferon in the in vitro cultivation of animal circular ssDNA virus such as Porcine Circovirus 2 or human TT virus in an animal cell line. Increased titres of animal circular ssDNA virus are obtained by addition of interferons or agents which ensure the production of endogenous interferons by said cell line and/or by the reduction of endosomal-lysosomal system acidification.

What is claimed is: 
     
         1 - 30 . (canceled) 
     
     
         31 . A method of improving the immune response of a subject to a vaccine comprising an attenuated animal circular ssDNA virus, said method comprising, administering one or more inhibitors of endosomal-lysosomal system acidification to said subject, simultaneously with or shortly before or after administering said vaccine to said subject. 
     
     
         32 . The method of  claim 31 , which further comprises administering interferon to said subject, simultaneously with or shortly before or after administering said vaccine. 
     
     
         33 . The method according to  claim 31 , wherein said animal circular ssDNA virus is a Circovirus. 
     
     
         34 . A vaccine comprising an attenuated animal circular ssDNA virus and one or more inhibitors of endosomal-lysosomal system acidification. 
     
     
         35 . The vaccine of  claim 34 , which further comprises an interferon. 
     
     
         36 . A vaccine comprising an attenuated animal circularssDNA virus and interferon. 
     
     
         37 . An undiluted medium of an in vitro culture of a cell-line comprising animal circular ssDNA virus characterized in that it further comprises at least 2 U/ml interferon. 
     
     
         38 . An undiluted medium of an in vitro culture of a cell-line comprising animal circular ssDNA virus characterized in that it further comprises an agent capable of inhibiting endosomal-lysosomal system acidification.