Novel peptides and uses thereof

Abstract

The present disclosure relates to novel peptides and their uses including, proline-rich peptides that are useful for displaying a protein of interest at the surfaces of a host cell such as a yeast. Polynucleotides, proteins, vectors and host cells that comprise or encode the novel proline-rich peptides, including libraries comprising such polynucleotides, proteins, vectors and/or host cells that comprise or encode novel proline-rich peptides are provided. Methods and materials for display and expression of proteins of interest are provided. Methods and materials are also provided by the present disclosure for isolating peptides capable of displaying a protein of interest (e.g., a marker protein), for generating libraries to display and/or express proteins of interest (e.g., antibodies such as humanized antibodies), for generating secretion vectors for such proteins of interest, and for generating proteins of interest (e.g., antibodies).

1 . A library of host cells displaying on their surface proteins of interest associated with a proline-rich peptide. 
     
     
         2 . The library of host cells of  claim 1 , wherein the host cells are yeast. 
     
     
         3 . The library of host cells of  claim 1 , wherein the protein of interest is an antibody or fragment thereof. 
     
     
         4 . (canceled) 
     
     
         5 . The library of host cells of  claim 1 , wherein the proline-rich peptide is an amino acid sequence comprising any of the amino acid sequences depicted in SEQ ID NOS: 1-116. 
     
     
         6 - 12 . (canceled) 
     
     
         13 . A library of host cells comprising polynucleotides coding for a protein of interest and a proline-rich peptide. 
     
     
         14 . The library of host cells of  claim 13 , wherein the host cells are yeast 
     
     
         15 . The library of host cells of  claim 13 , wherein the protein of interest is an antibody or binding fragment thereof. 
     
     
         16 . (canceled) 
     
     
         17 . The library of host cells of  claim 13 , wherein the proline-rich peptide is an amino acid sequence comprising PPP or any of the amino acid sequences depicted in SEQ ID NOS: 1-116. 
     
     
         18 - 27 . (canceled) 
     
     
         28 . A nucleic acid library comprising a plurality of polynucleotides coding for proteins of interest and a proline-rich peptide. 
     
     
         29 . The nucleic acid library of  claim 28 , wherein the protein of interest is an antibody or binding fragment thereof. 
     
     
         30 . (canceled) 
     
     
         31 . The nucleic acid library of  claim 28 , wherein the proline-rich peptide is an amino acid sequence comprising PPP or any of the amino acid sequences depicted in SEQ ID NOS: 1-116. 
     
     
         32 - 103 . (canceled) 
     
     
         104 . A method of displaying a protein of interest associated with a proline-rich peptide on a surface of a host cell, the method comprising: culturing a host cell comprising a polynucleotide coding for a protein of interest and a polynucleotide coding for a proline-rich peptide under conditions wherein the polynucleotide sequences are expressed and the protein of interest and the proline-rich peptide are displayed. 
     
     
         105 - 106 . (canceled) 
     
     
         107 . The method of  claim 104 , wherein the protein of interest is an antibody or binding fragment thereof. 
     
     
         108 . (canceled) 
     
     
         109 . The method of  claim 104 , wherein the proline-rich peptide is an amino acid sequence comprising PPP or any of the amino acid sequences depicted in SEQ ID NOS: 1-116. 
     
     
         110 - 116 . (canceled) 
     
     
         117 . The method of  claim 104 , wherein the host cell is a yeast cell. 
     
     
         118 - 301 . (canceled)