US2013330307A1PendingUtilityA1

Feed additive composition

41
Assignee: MILLAN LUIS FERNANDO ROMEROPriority: Feb 18, 2011Filed: Jan 19, 2012Published: Dec 12, 2013
Est. expiryFeb 18, 2031(~4.6 yrs left)· nominal 20-yr term from priority
Inventors:Luis Fernando Romero Millan
A61P 33/02A61P 29/00A61K 35/745A23K 50/30C12N 9/16A61K 35/742A61K 35/744A23K 20/189A23K 40/10A23K 50/75A23K 10/18C12N 1/20A23K 50/60C12Y 301/03026A61K 38/465A23K 40/30A61K 35/741A61K 38/48A61K 35/747A61P 1/00C12R 2001/125C12N 1/205C12N 9/50A23K 1/009A23K 20/00
41
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Claims

Abstract

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

Claims

exact text as granted — not AI-modified
1 . A feed additive composition comprising a direct fed microbial in combination with a protease and a phytase. 
     
     
         2 . A feed additive composition according to  claim 1  wherein the direct fed microbial is an antipathogen direct fed microbial. 
     
     
         3 . A feed additive composition according to  claim 1  or  claim 2  wherein the direct fed microbial is a viable bacterium. 
     
     
         4 . A feed additive composition according to any one of  claims 1  to  3  wherein the direct fed microbial comprises a bacterium from one or more of the following genera:  Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium  and  Megasphaera  and combinations thereof. 
     
     
         5 . A feed additive composition according to any one of the preceding claims wherein the direct fed microbial comprises a bacterium from one or more of the following species:  Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus  spp, and  Pediococcus  spp,  Lactobacillus  spp,  Bifidobacterium  spp,  Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis  ssp.  animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius  ssp.  salivarius, Megasphaera elsdenii, Propionibacteria  sp and combinations thereof. 
     
     
         6 . A feed additive composition according to any one of the preceding claims wherein the direct fed microbial is one or more of the following strains:  Bacillus subtilis  strains 3A-P4 (PTA-6506); 15A-P4 (PTA-6507); 22C-P1 (PTA-6508); 2084 (NRRL B-500130); LSSA01 (NRRL-B-50104); BS27 (NRRL B-50105); BS 18 (NRRL B-50633); and BS 278 (NRRL B-50634). 
     
     
         7 . A feed additive composition according to any one of the preceding claims wherein the direct fed microbial is in the form of an endospore. 
     
     
         8 . A feed additive composition according to any one of the preceding claims wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a  Nocardiopsis  protease. 
     
     
         9 . A feed additive composition according to any one of the preceding claims wherein the phytase is a 6-phytase or a 3-phytase. 
     
     
         10 . A feed additive composition according to  claim 9  wherein the phytase is a 6-phytase. 
     
     
         11 . A feed additive composition according to any one of the preceding claims wherein the phytase is an  E. coli  phytase or a  Buttiauxella  phytase or  Hafnia  phytase or  Citrobacter  phytase or an  Aspergillus  phytase or a  Penicillium  phytase or a  Trichoderma  phytase or an  E. coli  phytase or a  Hansenula  phytase. 
     
     
         12 . A feed additive composition according to any one of the preceding claims wherein the phytase is present at a dosage of between 200 FTU/g feed additive composition and 10000 FTU/g feed additive composition. 
     
     
         13 . A feed additive composition according to any one of the preceding claims wherein the protease is present at a dosage of 1000 PU/g feed additive composition and 60000 PU/g feed additive composition. 
     
     
         14 . A feed additive composition according to any one of the preceding claims wherein the DFM is present at a dosage of 3.75×10 7 CFU/g feed additive composition and 1×10 11 CFU/g feed additive composition. 
     
     
         15 . A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase. 
     
     
         16 . A method according to  claim 15  comprising administering the feed additive composition according to any one of  claims 1 - 14 . 
     
     
         17 . A method according to  claim 15  or  16  wherein the direct fed microbial is an antipathogen direct fed microbial. 
     
     
         18 . A method according to any one of  claims 15 - 17  wherein the direct fed microbial is a viable bacterium. 
     
     
         19 . A method according to any one of  claims 15  to  18  wherein the direct fed microbial comprises a bacterium from one or more of the following genera:  Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium  and  Megasphaera  and combinations thereof. 
     
     
         20 . A method according to any one of  claims 15 - 19  wherein the direct fed microbial comprises a bacterium from one or more of the following species:  Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus  spp, and  Pediococcus  spp,  Lactobacillus  spp,  Bifidobacterium  spp,  Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis  ssp.  animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius  ssp.  salivarius, Megasphaera elsdenii, Propionibacteria  sp and combinations thereof. 
     
     
         21 . A method according to any one of  claims 15 - 20  wherein the direct fed microbial comprises a bacterium is one or more of the following strains:  Bacillus subtilis  strains 3A-P4 (PTA-6506); 15A-P4 (PTA-6507); 22C-P1 (PTA-6508); 2084 (NRRL B-500130); LSSA01 (NRRL-B-50104); BS27 (NRRL B-50105); BS 18 (NRRL B-50633); and BS 278 (NRRL B-50634). 
     
     
         22 . A method according to any one of  claims 15 - 21  wherein the direct fed microbial is in the form of an endospore. 
     
     
         23 . A method according any one of  claims 15 - 22  wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a  Nocardiopsis  protease. 
     
     
         24 . A method according any one of  claims 15 - 23  wherein the phytase is a 6-phytase or a 3-phytase. 
     
     
         25 . A method according to  claim 24  wherein the phytase is a 6-phytase. 
     
     
         26 . A method according any one of  claims 15 - 25  wherein the phytase is an  E. coli  phytase or a  Buttiauxella  phytase or  Hafnia  phytase or  Citrobacter  phytase or an  Aspergillus  phytase or a  Penicillium  phytase or a  Trichoderma  phytase or an  E. coli  phytase or a  Hansenula  phytase. 
     
     
         27 . Use of a direct fed microbial in combination with a protease and a phytase for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility) or for improving nitrogen retention) or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject or for reducing nutrient excretion in manure. 
     
     
         28 . Use according to  claim 27  wherein the feed additive composition according to any one of  claims 1 - 14  is used. 
     
     
         29 . Use according to any one of  claims 27 - 28  wherein the direct fed microbial is an antipathogen direct fed microbial. 
     
     
         30 . Use according to any one of  claims 27 - 29  wherein the direct fed microbial is a viable bacterium. 
     
     
         31 . Use according to any one of  claims 27 - 30  wherein the direct fed microbial comprises a bacterium from one or more of the following genera:  Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium  and  Megasphaera  and combinations thereof. 
     
     
         32 . Use according to any one of  claims 27 - 31  wherein the direct fed microbial comprises a bacterium from one or more of the following species:  Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus  spp, and  Pediococcus  spp,  Lactobacillus  spp,  Bifidobacterium  spp,  Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis  ssp.  animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius  ssp.  salivarius, Megasphaera elsdenii, Propionibacteria  sp and combinations thereof. 
     
     
         33 . Use according to any one of  claims 27 - 32  wherein the direct fed microbial is one or more of the following strains:  Bacillus subtilis  strains 3A-P4 (PTA-6506); 15A-P4 (PTA-6507); 22C-P1 (PTA-6508); 2084 (NRRL B-500130); LSSA01 (NRRL-B-50104); BS27 (NRRL B-50105); BS 18 (NRRL B-50633); and BS 278 (NRRL B-50634). 
     
     
         34 . Use according to any one of  claims 27 - 33  wherein the direct fed microbial is in the form of an endospore. 
     
     
         35 . Use according to any one of  claims 27 - 34  wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a  Nocardiopsis  protease. 
     
     
         36 . Use according to any one of  claims 27 - 35  wherein the phytase is a 6-phytase or a 3-phytase. 
     
     
         37 . Use according to  claim 36  wherein the phytase is a 6-phytase. 
     
     
         38 . Use according to any one of  claims 27 - 37  wherein the phytase is an  E. coli  phytase or a  Buttiauxella  phytase or  Hafnia  phytase or  Citrobacter  phytase or an  Aspergillus  phytase or a  Penicillium  phytase or a  Trichoderma  phytase or an  E. coli  phytase or a  Hansenula  phytase. 
     
     
         39 . A kit comprising a direct fed microbial, a protease, a phytase and instructions for administration. 
     
     
         40 . A kit according to  claim 39  wherein said kit comprises the feed additive composition according to any one of  claims 1 - 14 . 
     
     
         41 . A kit according to any one of  claims 39 - 40  wherein the direct fed microbial is an antipathogen direct fed microbial. 
     
     
         42 . A kit according to any one of  claims 39 - 41  wherein the direct fed microbial is a viable bacterium. 
     
     
         43 . A kit according to any one of  claims 39 - 42  wherein the direct fed microbial comprises a bacterium from one or more of the following genera:  Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium  and  Megasphaera  and combinations thereof. 
     
     
         44 . A kit according to any one of  claims 39 - 43  wherein the direct fed microbial comprises a bacterium from one or more of the following species:  Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus  spp, and  Pediococcus  spp,  Lactobacillus  spp,  Bifidobacterium  spp,  Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis  ssp.  animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius  ssp.  salivarius, Megasphaera elsdenii, Propionibacteria  sp and combinations thereof. 
     
     
         45 . A kit according to any one of  claims 39 - 43  wherein the direct fed microbial is one or more of the following strains:  Bacillus subtilis  strains 3A-P4 (PTA-6506); 15A-P4 (PTA-6507); 22C-P1 (PTA-6508); 2084 (NRRL B-500130); LSSA01 (NRRL-B-50104); BS27 (NRRL B-50105); BS 18 (NRRL B-50633); and BS 278 (NRRL B-50634). 
     
     
         46 . A kit according to any one of  claims 39 - 45  wherein the direct fed microbial is in the form of an endospore. 
     
     
         47 . A kit according to any one of  claims 39 - 46  wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a  Nocardiopsis  protease. 
     
     
         48 . A kit according to any one of  claims 39 - 47  wherein the phytase is a 6-phytase or a 3-phytase. 
     
     
         49 . A kit according to  claim 48  wherein the phytase is a 6-phytase. 
     
     
         50 . A kit according to any one of  claims 39 - 49  wherein the phytase is an  E. coli  phytase or a  Buttiauxella  phytase or  Hafnia  phytase or  Citrobacter  phytase or an  Aspergillus  phytase or a  Penicillium  phytase or a  Trichoderma  phytase or an  E. coli  phytase or a  Hansenula  phytase. 
     
     
         51 . A method of preparing a feed additive composition, comprising admixing a direct fed microbial with a protease and a phytase and (optionally) packaging. 
     
     
         52 . A feed comprising a feed additive composition according to any one of  claims 1 - 14 . 
     
     
         53 . A feed according to  claim 52  wherein the phytase is present at a dosage of between 400 FTU/kg feed and 1000 FTU/kg feed. 
     
     
         54 . A feed according to any one of  claims 52 - 53  wherein the protease is present at a dosage of between 2000 PU/kg feed to 6000 PU/kg feed. 
     
     
         55 . A feed according to any one of  claims 52 - 54  wherein the DFM is present at a dosage of 7.5×10 4 CFU/kg feed and 1×10 7 CFU/kg feed. 
     
     
         56 . A method of preparing a feedstuff comprising admixing a feed component with a feed additive composition according to any one of  claims 1 - 14 . 
     
     
         57 . A premix comprising a feed additive composition comprising a direct fed microbial in combination with a protease and a phytase, and at least one mineral and/or at least one vitamin. 
     
     
         58 . A premix comprising a feed additive composition according to any one of  claims 1 - 14  in combination with at least one mineral and/or at least one vitamin. 
     
     
         59 . A feed additive composition according to any one of  claims 1 - 14  for preventing and/or treating coccidiosis and/or necrotic enteritis in a subject. 
     
     
         60 . A method of preventing and/or treating necrotic enteritis and/or coccidiosis wherein an effective amount of a feed additive composition according to any one of  claims 1 - 14  is administered to a subject. 
     
     
         61 . A feed additive composition or feed or kit or method or use or premix as defined generally herein with reference to the Figures and the Examples.

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