US2013315967A1PendingUtilityA1

Antagonistic properties of reef fish microflora

36
Individually held — no corporate assignee on recordPriority: Apr 30, 2004Filed: Jul 29, 2013Published: Nov 28, 2013
Est. expiryApr 30, 2024(expired)· nominal 20-yr term from priority
Inventors:Cynthia K. Bruno
A61P 31/04A61P 31/00C09D 5/1606C09D 5/14C12R 2001/01C12N 1/205C09D 5/1687A01N 63/20A01N 63/02
36
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

The present invention provides methods for preventing biofilm formation on a surface. The present invention also relates to anti-biofilm forming agents, to methods of producing and using them, and to anti-fouling coatings produced therefrom.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . Bacteria isolated from the epithelial mucosal surfaces of coral reef fish, wherein said isolated bacteria inhibit one or more organisms selected from the group consisting of  Staphylococcus aureus, Staphylococcus saprophiticus, Staphylococcus epidermidis, Micrococcus  spp.,  Enterobacter cloacae, Serratia marcescens, Shigella sonnei, Klebsiella pneumoniae, Proteus vulgaris, Salmonella typhimurium , a photosynthetic eukaryotic organism and a non-photosynthetic eukaryotic organism. 
     
     
         2 . The bacteria of  claim 1 , wherein the coral reef fish is selected from the group consisting of  Sparisoma ninidae  (Parrot Fish) and  Lutjanus purpureus  (Red Snapper). 
     
     
         3 . A bacterial isolate selected from the group consisting of P3-2 (PTA-6763), P4-4 (PTA-6682), P5-2 (PTA-6764), P2-2 (PTA-6681), P6-5 (PTA-6765) and P6-6 (PTA-6766). 
     
     
         4 . The isolated bacteria  claim 1 , wherein the isolated bacteria are closest related to a bacteria selected from the group consisting of  Staphylococcus warneri, Psychrobacter immobilis, Aerococcus viridans, Desemzia incerta , a  Salmonella  spp. and a  Vibrio  spp. 
     
     
         5 . A method of using the isolated bacteria of  claim 1 , wherein said method comprises inhibiting one or more target organisms selected from the group consisting of bacteria, a photosynthetic eukaryotic organism and a non-photosynthetic eukaryotic organism. 
     
     
         6 . The method of  claim 5 , wherein the inhibition involves adversely affecting the physiological or morphological characteristics of the target organism. 
     
     
         7 . The method of  claim 6 , wherein the target organism is pathogenic to humans. 
     
     
         8 . The method of  claim 6 , wherein the target organism is naturally or normally capable of growing in or on a biofilm. 
     
     
         9 . The method of  claim 5 , wherein the isolated bacteria inhibit, prevent, or otherwise interfere with the colonization of a biofilm by the target organism or inhibit, prevent, or otherwise interfere with the ability of the target organism to grow in or on a biofilm. 
     
     
         10 . The method of  claim 5 , wherein the target organism is an antibiotic resistant pathogen. 
     
     
         11 . An isolated extract obtained from bacteria found on the epithelial mucosal surfaces of coral reef fish, wherein said isolated extract inhibits one or more organisms selected from the group consisting of  Staphylococcus aureus, Staphylococcus saprophiticus, Staphylococcus epidermidis, Micrococcus  spp.,  Enterobacter cloacae, Serratia marcescens, Shigella sonnei, Klebsiella pneumoniae, Proteus vulgaris, Salmonella typhimurium , a photosynthetic eukaryotic organism and a non-photosynthetic eukaryotic organism. 
     
     
         12 . The isolated extract of  claim 11 , wherein the coral reef fish is selected from the group consisting of  Sparisoma ninidae  (Parrot Fish) and  Lutjanus purpureus (Red Snapper). 
     
     
         13 . The isolated extract of  claim 11 , wherein the bacteria is selected from the group consisting of P3-2 (PTA-6763), P4-4 (PTA-6682), P5-2 (PTA-6764), P2-2 (PTA-6681), P6-5 (PTA-6765) and P6-6 (PTA-6766). 
     
     
         14 . The isolated extract of  claim 11 , wherein the bacteria are closest related to a bacteria selected from the group consisting of  Staphylococcus warneri, Psychrobacter immobilis, Aerococcus viridans, Desemzia incerta , a  Salmonella  spp. and a  Vibrio  spp. 
     
     
         15 . A method of using the isolated extract of  claim 11 , wherein said method comprises inhibiting one or more target organisms selected from the group consisting of bacteria, a photosynthetic eukaryotic organism and a non-photosynthetic organism. 
     
     
         16 . The method of  claim 15 , wherein the inhibition involves adversely affecting the physiological or morphological characteristics of the target organism. 
     
     
         17 . The method of  claim 15 , wherein the target organism is pathogenic to humans. 
     
     
         18 . The method of  claim 15 , wherein the target organism is naturally or normally capable of growing in or on a biofilm. 
     
     
         19 . The method of  claim 15 , wherein the isolated bacteria inhibit, prevent, or otherwise interfere with the colonization of a biofilm by the target organism or inhibit, prevent, or otherwise interfere with the ability of the target organism to grow in or on a biofilm. 
     
     
         20 . The method of  claim 15 , wherein the target organism is an antibiotic resistant pathogen. 
     
     
         21 . A method for reducing fouling of a marine surface, comprising coating the marine surface with a anti-fouling composition comprising the isolated bacteria of  claim 1  or the isolated extracts obtained from said bacteria. 
     
     
         22 . A marine anti-fouling paint comprising: a paint base material, suitable for marine applications, wherein the base material is selected from the group consisting of an epoxy material, a polyurethane material, a fiberglass material, a polyester material, a silicone material, and an acrylic material; a pigment; and at least one isolated bacteria of  claim 1  mixed with the paint base material, wherein the isolated bacteria are present in an effective amount to reduce or prevent fouling of a marine surface coated with the marine anti-fouling paint. 
     
     
         23 . A marine anti-fouling paint comprising: a paint base material, suitable for marine applications, wherein the base material is selected from the group consisting of an epoxy material, a polyurethane material, a fiberglass material, a polyester material, a silicone material, and an acrylic material; a pigment; and at least one isolated extract of  claim 11  mixed with the paint base material, wherein the isolated bacteria are present in an effective amount to reduce or prevent fouling of a marine surface coated with the marine anti-fouling paint. 
     
     
         24 . A method for reducing the coefficient of drag of a marine surface comprising coating the surface with a paint as claimed in  claim 22 . 
     
     
         25 . An indwelling medical device having one or more of its surfaces coated or impregnated with an anti-biofilm composition comprising at least one isolated extract of  claim 11 , wherein the isolated extract is present in an effective amount to inhibit a target organism selected from the group consisting of bacteria, photosynthetic eukaryotic organisms and non-photosynthetic eukaryotic organisms. 
     
     
         26 . A method for inhibiting  Staphylococcus  microbial growth on surfaces of an indwelling medical device comprising: applying to a surface of the medical device a coating of an anti-biofilm composition comprising at least one isolated extract of  claim 11 .

Join the waitlist — get patent alerts

Track US2013315967A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.