Compound

Abstract

A compound including a polymer is represented by general formula (1): L-Y-A  (1) wherein, A is a single-chain antibody moiety, which is a polypeptide including an antigen-binding site, L is a linker moiety, which is a polypeptide including a protease cleavage site, Y is a peptide moiety, which includes 0 or more amino acids connecting the linker moiety L with the single-chain antibody moiety A, and the linker moiety L binds to an N terminus of the peptide moiety Y or an N terminus of the single-chain antibody moiety A.

What is claimed is: 
     
         1 . A process for detecting a lesion marker, comprising administering to an individual a compound comprising a polymer represented by formula (1) to a single-chain antibody moiety of which a signal generating molecule is linked or adding such a compound to a sample obtained from an individual and detecting signals, thereby detecting presence or absence of lesion markers or presence or absence of lesion sites generating lesion markers in the individual or in the sample obtained from an individual,
 wherein the compound comprising the polymer represented by general formula (1) is as follows:
   L-Y-A  (1)
 
   wherein, A is a single-chain antibody moiety, which is a polypeptide comprising an antigen-binding site; L is a linker moiety, which is a polypeptide comprising a protease cleavage site; Y is a peptide moiety, which comprises 0 or more amino acids connecting the linker moiety L with the single-chain antibody moiety A; and the linker moiety L binds to an N terminus of the peptide moiety Y or an N terminus of the single-chain antibody moiety A.   
     
     
         2 . A process for producing a compound comprising a polymers represented by general formula (1), comprising inserting to a plasmid a nucleic acid which has a base sequence encoding a single-chain antibody moiety A, a base sequence encoding a linker moiety L and a base sequence encoding a peptide moiety Y; introducing to bacteria the plasmid in which the nucleic acid is inserted; and collecting a compound expressed by the bacteria to which the plasmid is introduced:
   L-Y-A  (1)
   wherein, A is single-chain antibody moiety, which is a polypeptide comprising an antigen-binding site; L is a linker moiety, which is a polypeptide comprising a protease cleavage site; Y is a peptide moiety, which comprises 0 or more amino acids connecting the linker moiety L with the single-chain antibody moiety A; and the linker moiety L binds to an N terminus of the peptide moiety Y or an N terminus of the single-chain antibody moiety A.