Compositions and methods of producing enterokinase in yeast

Abstract

The present specification disclose polynucleotide molecules encoding an enterokinase, yeast expression constructs including a yeast expression vector and a polynucleotide molecules encoding an enterokinase, yeast cells comprising such a yeast expression construct, methods of producing enterokinase using such yeast cells, and method of cleaving or preparing a recombinant polypeptide using an enterokinase produced by such methods.

1 . A method of cleaving a recombinant polypeptide, the method comprising the step contacting a polypeptide comprising a cleavage site of SEQ ID NO:1 with an enterokinase, wherein the enterokinase is produced by expressing in a yeast cell comprising a yeast expression construct comprising SEQ ID NO:4, wherein contacting the recombinant polypeptide with the enterokinase results in specific cleavage of SEQ ID NO:1. 
     
     
         2 . A method of cleaving a recombinant polypeptide, the method comprising the step contacting a polypeptide comprising a cleavage site of SEQ ID NO:1 with an enterokinase, wherein the enterokinase is produced by expressing in a yeast cell comprising a yeast expression construct comprising SEQ ID NO:6, wherein contacting the recombinant polypeptide with the enterokinase results in specific cleavage of SEQ ID NO:1.