Method for producing biodiesel

Abstract

The present disclosure is directed to the method for producing the biodiesel comprising steps of providing a biomass having an oil, obtaining a crude mixture of the biomass, and then performing a transesterification in which the crude mixture is used as a reacting material thereof to produce the biodiesel.

What is claimed is: 
     
         1 . A method for producing a biodiesel, comprising steps of:
 providing a microalga;   disrupting the microalga to obtain a disrupted microalga; and   mixing the disrupted microalga directly with a catalyst, an alcohol and a co-solvent to produce the biodiesel.   
     
     
         2 . The method as claimed in  claim 1 , wherein the disrupted microalga has all of ingredients of the microalga, the all of ingredients include a microalgal oil and a residue, the microalgal oil and the alcohol are reacted in a transesterification, and the catalyst is used to promote the transesterification of the microalgal oil and the alcohol, and is one selected from the group consisting of a lipase, an acid catalyst, a base catalyst and a combination thereof. 
     
     
         3 . The method as claimed in  claim 2 , wherein the catalyst is an immobilized lipase. 
     
     
         4 . The method as claimed in  claim 4 , wherein the immobilized lipase includes a particle and a lipase of  Burkholderia  sp. C20 immobilized thereon. 
     
     
         5 . A biodiesel mixture having the biodiesel produced by the method as claimed in  claim 2  and the residue. 
     
     
         6 . The method as claimed in  claim 1 , wherein the disrupted microalga is directly mixed with the catalyst, the alcohol and the co-solvent without removing any ingredient therefrom after the step of disrupting the microalga to obtain the disrupted microalga. 
     
     
         7 . The method as claimed in  claim 1 , wherein the alcohol is a short chain alcohol being one selected from the group consisting of a methanol, an ethanol, a propanol, a butanol, a pentanol and a combination thereof, and the co-solvent is one selected from the group consisting of a hexane, an acetone, a chloroform, a petroleum ether and a combination thereof. 
     
     
         8 . The method as claimed in  claim 1 , wherein the microalga has a species of  Chlorella vulgaris  ESP-31. 
     
     
         9 . The method as claimed in  claim 1 , wherein the microalga is one selected from the group consisting of a dry microalga, a wet microalga and a combination thereof. 
     
     
         10 . A method for producing a biodiesel, comprising steps of:
 providing a microalga;   disrupting the microalga to obtain a disrupted microalga; and   performing a transesterification in which the disrupted microalga is directly used as a reacting material thereof to produce the biodiesel.   
     
     
         11 . The method as claimed in  claim 10  further comprising a step of directly mixing the disrupted microalga with a catalyst and an alcohol to perform the transesterification, wherein the disrupted microalga has whole ingredients of the microalga, the whole ingredients include a microalgal oil and a residue, the microalgal oil and the alcohol are reacted in the transesterification, and the catalyst is used to promote the transesterification of the microalgal oil and the alcohol. 
     
     
         12 . The method as claimed in  claim 11 , wherein the disrupted microalga mixed with the catalyst and the alcohol is further mixed with a co-solvent for promoting the transesterification, the catalyst is one selected from the group consisting of a lipase, an acid catalyst, a base catalyst and a combination thereof, the alcohol is a short chain alcohol being one selected from the group consisting of a methanol, an ethanol, a propanol, a butanol, a pentanol and a combination thereof, and the co-solvent is one selected from the group consisting of a hexane, an acetone, a chloroform, a petroleum ether and a combination thereof. 
     
     
         13 . The method as claimed in  claim 11 , wherein the catalyst is an immobilized lipase including a particle and a lipase of  Burkholderia  sp. C20 immobilized thereon. 
     
     
         14 . A biodiesel mixture having the biodiesel produced by the method as claimed in  claim 11  and the residue. 
     
     
         15 . A method for producing a biodiesel, comprising steps of:
 providing a biomass having an oil;   obtaining a crude mixture from the biomass; and   performing a transesterification in which the crude mixture is used as a reacting material thereof to produce the biodiesel.   
     
     
         16 . The method as claimed in  claim 15 , wherein the crude mixture is obtained by disrupting the biomass, and the method further comprises a step of directly mixing the crude mixture with a catalyst and an alcohol without removing any ingredient from the crude mixture to perform the transesterification. 
     
     
         17 . The method as claimed in  claim 15  further comprising a step of directly mixing the crude mixture with a catalyst and an alcohol to perform the transesterification, wherein the crude mixture has whole ingredients of the biomass, the whole ingredients have an oil and a residue, the oil and the alcohol are reacted in the transesterification, and the catalyst is used to promote the transesterification of the oil and the alcohol. 
     
     
         18 . The method as claimed in  claim 17 , wherein the catalyst is an immobilized lipase including a particle and a lipase of  Burkholderia  sp. C20 immobilized thereon. 
     
     
         19 . A biodiesel mixture having the biodiesel produced by the method as claimed in  claim 17  and the residue. 
     
     
         20 . The method as claimed in  claim 15 , wherein the biomass is a microalgae biomass having a species of  Chlorella vulgaris  ESP-31.