Methods and Systems for Saccharification of Biomass

Abstract

Provided are methods and compositions for high yields while using reduced enzyme loads in saccharification and fermentation processes. These methods increase the efficiency of enzymes and result in improved yields and composition of saccharification and fermentation end products.

1 . A method of producing one or more fermentation end-products from saccharide polymers obtained from a biomass composition comprising cellulose, hemicellulose, and/or lignocellulose, the method comprising:
 (a) pretreating the biomass composition comprising cellulose, hemicellulose, and/or lignocellulose to produce a pretreated biomass composition comprising solid particles that are less than 1.5 mm in size and a first population of saccharide polymers, wherein pretreating comprises:
 (i) hydrating the biomass composition in an aqueous medium to produce a hydrated biomass composition, 
 (ii) reducing the hydrated biomass composition by mechanical size reduction to produce solid particles that are less than 1.5 mm in size, and 
 (iii) heating the hydrated biomass composition for a time sufficient to produce the pretreated biomass composition comprising the solid particles that are less than 1.5 mm in size and the first population of saccharide polymers; and 
   (b) hydrolyzing and fermenting the first population of saccharide polymers with one or more microorganisms for a time sufficient to produce the one or more fermentation end-products.   
     
     
         2 . The method of  claim 1 , wherein hydrolyzing and fermenting further comprises treating with one or more enzymes. 
     
     
         3 . The method of  claim 1 , wherein the first population of saccharide polymers are soluble. 
     
     
         4 . The method of  claim 2 , wherein the one or more enzymes comprise one or more hemicellulases, one or more cellulases, or a combination thereof. 
     
     
         5 . The method of  claim 2 , wherein the one or more enzymes are at a total level from about 1% to about 20% w/w by dry biomass weight. 
     
     
         6 . The method of  claim 1 , wherein at least 50% of the solid particles in the pretreated biomass composition are from about 0.1 mm to about 1 mm in size. 
     
     
         7 . The method of  claim 1 , wherein all of the solid particles in the pretreated biomass are less than 1 mm in size. 
     
     
         8 . The method of  claim 1 , wherein the first population of saccharide polymers in the pretreated biomass composition comprises oligosaccharides. 
     
     
         9 . The method of  claim 1 , wherein mechanical size reduction comprises cutting, steam injection, steam explosion, acid-catalyzed steam explosion, ammonia fiber/freeze explosion (AFEX), or a combination thereof. 
     
     
         10 . The method of  claim 1 , wherein mechanical size reduction comprises cutting with a first rotating cutter and/or a second rotating cutter. 
     
     
         11 . The method of  claim 1 , wherein pretreating the biomass composition further comprises dewatering the hydrated biomass composition to from about 10% to about 40% solids by dry biomass weight prior to hydrolyzing. 
     
     
         12 . The method of  claim 1 , wherein the first population of saccharide polymers comprises disaccharides, trisaccharides, tetrasaccharides, pentasaccharides, hexasaccharides, heptasaccharides, octasaccharides, enneasaccharides, decasaccharides, or a combination thereof. 
     
     
         13 . The method of  claim 1 , wherein pretreating the biomass composition further comprises hydrolyzing the hydrated biomass composition with one or more enzymes. 
     
     
         14 . The method of  claim 13 , wherein the one or more enzymes comprise one or more endocellulases, one or more exocellulases, one or more endo-hemicellulases, one or more exo-hemicellulases, or a combination thereof. 
     
     
         15 . The method of  claim 13 , wherein the one or more enzymes are present at a total level of about 0.1% w/w to about 20% w/w by dry biomass weight. 
     
     
         16 . The method of  claim 1 , further comprising separating the solid particles of the pretreated biomass composition from the first population of saccharide polymers prior to hydrolyzing and fermenting. 
     
     
         17 . The method of  claim 16 , wherein the solid particles are hydrolyzed with an acid or one or more enzymes to produce a second population of saccharide polymers. 
     
     
         18 . The method of  claim 1 , wherein the first population of saccharide polymers comprises glucose residues, xylose residues, mannose residues, galactose residues, rhamnose residues, arabinose residues, or a combination thereof. 
     
     
         19 . The method of  claim 17 , wherein the second population of saccharide polymers comprises glucose residues. 
     
     
         20 . The method of  claim 17 , wherein the second population of saccharide polymers comprises oligosaccharides. 
     
     
         21 . The method of  claim 17 , wherein the second population of saccharide polymers is soluble. 
     
     
         22 . A method of producing one or more fermentation end-products from a biomass composition comprising cellulose, hemicellulose, and/or lignocellulose, the method comprising:
 (a) hydrating the biomass composition in an aqueous medium;   (b) reducing the biomass composition by a first mechanical size reduction;   (c) dewatering the biomass composition;   (d) reducing the biomass composition by a second mechanical size reduction to produce solid particles that are less than 1.5 mm in size;   (e) hydrolyzing the biomass composition by heating the biomass composition for a time sufficient to produce saccharide polymers;   (f) hydrolyzing the saccharide polymers with one or more first microorganisms and, optionally, one or more enzymes to produce monosaccharides, and/or disaccharides; and   (g) fermenting the monosaccharides, and/or disaccharides with one or more second microorganisms to produce the one or more fermentation end-products.   
     
     
         23 . The method of  claim 22 , wherein hydrolyzing the biomass composition further comprises adding one or more enzymes. 
     
     
         24 . The method of  claim 23 , wherein the one or more enzymes are present at a total level of about 0.1% w/w to about 20% w/w by dry biomass weight. 
     
     
         25 . The method of  claim 22 , wherein at least 50% of the solid particles in the pretreated biomass composition are from about 0.1 mm to about 1 mm in size. 
     
     
         26 . A composition comprising one or more fermentation end-products produced by the method of  claim 22 . 
     
     
         27 . The method of  claim 22 , wherein hydrolyzing the saccharide polymers and fermenting the monosaccharides and/or disaccharides are performed simultaneously. 
     
     
         28 . The method of  claim 22 , wherein hydrolyzing the saccharide polymers and fermenting the monosaccharides and/or disaccharides are performed sequentially. 
     
     
         29 . The method of  claim 22 , wherein the one or more first microorganisms are the same as the one or more second microorganisms. 
     
     
         30 . A method of producing a first and second population of saccharide polymers from a biomass composition comprising cellulose, hemicellulose, and/or lignocellulose, the method comprising:
 (a) pretreating the biomass composition comprising cellulose, hemicellulose, and/or lignocellulose to produce a pretreated biomass composition comprising solid particles that are less than 1.5 mm in size and a first population of saccharide polymers, wherein pretreating comprises:
 (i) hydrating the biomass composition in an aqueous medium to produce a hydrated biomass composition; 
 (ii) reducing the hydrated biomass composition by mechanical size reduction to produce solid particles that are less than 1.5 mm in size, and 
 (iii) heating the hydrated biomass composition for a time sufficient to produce the pretreated biomass composition comprising the solid particles that are less than 1.5 mm in size and the first population of saccharide polymers; and 
   (b) hydrolyzing the solid particles for a time sufficient to produce a second population of saccharide polymers.