Detection of Degradation Products of Feline NT-proBNP

Abstract

A method for determining the amount of NT-proBNP in blood samples from felines. The method includes detecting degradation products of feline NT-proBNP by various methods, including using antibodies, kits and devices.

What is claimed is: 
     
         1 . An immunoassay device comprising a solid support having a first antibody bound thereto a first antibody specific for a fragment of feline NT-proBNP selected from the group consisting of: 
       
         
           
                 
                 
               
                     
                   (SEQ ID NO: 5) 
                 
                     
                   ASEASAIQELLDGLRDTVSEL; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 9) 
                 
                     
                   LGPLQQGHSPAESWEAQEEPPARVLAPHDNVLR; 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 10) 
                 
                     
                   LQQGHSPAESWEAQEEPPARVLAPHDNVLR. 
                 
             
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         2 . The device of  claim 1 , wherein the first antibody is specific for SEQ ID NO: 5. 
     
     
         3 . The device of  claim 1 , wherein the first antibody is specific for SEQ ID NO:9. 
     
     
         4 . The device of  claim 1 , wherein the first antibody is specific for SEQ ID NO:10. 
     
     
         5 . The device of  claim 1 , wherein the first antibody is a monoclonal antibody. 
     
     
         6 . The device of  claim 5 , wherein the first antibody is raised against the fragment of feline NT-proBNP. 
     
     
         7 . The device of  claim 1 , wherein the first antibody is a polyclonal antibody. 
     
     
         8 . The device of  claim 7 , wherein the first antibody is raised the fragment of feline NT-proBNP. 
     
     
         9 . The device of  claim 1 , further comprising a second antibody being specific for the fragment of feline NT-proBNP. 
     
     
         10 . The device of  claim 9 , wherein the second antibody is a monoclonal antibody. 
     
     
         11 . The device of  claim 10 , wherein the monoclonal antibody is raised the fragment of feline NT-proBNP. 
     
     
         12 . The device of  claim 9 , wherein the second antibody is a polyclonal antibody. 
     
     
         13 . The device of  claim 12 , wherein the second antibody is raised the fragment of feline NT-proBNP. 
     
     
         14 . The device of  claim 9 , wherein the second antibody is labeled. 
     
     
         15 . The device of  claim 1 , further comprising a labeled detection reagent that competes with the fragment for binding with the first antibody. 
     
     
         16 . The device of  claim 15 , wherein the first antibody is specific for SEQ ID NO: 5. 
     
     
         17 . The device of  claim 15 , wherein the first antibody is specific for SEQ ID NO:9. 
     
     
         18 . The device of  claim 15 , wherein the first antibody is specific for SEQ ID NO:10. 
     
     
         19 . The device of  claim 15 , wherein the first antibody is a monoclonal antibody. 
     
     
         20 . The device of  claim 15 , wherein the first antibody is a polyclonal antibody.