Detection, isolation and uses of renalase (monoamine oxidase c)

Abstract

The present invention provides for the identification, isolation and uses of mammalian Monoamine Oxidase C (MAO-C), also known as renalase.

1 - 69 . (canceled) 
     
     
         70 . An isolated renalase protein capable of metabolizing catecholamines, comprising:
 an FAD-binding site with bound FAD cofactor and an amine oxidase domain, the renalase protein comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:2,   wherein the renalase protein is recombinantly produced in a host cell in the presence of exogenous flavin adenine dinucleotide (FAD).   
     
     
         71 . The renalase protein of  claim 70 , wherein the renalase protein comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2. 
     
     
         72 . The renalase protein of  claim 70 , wherein the renalase protein comprises an amino acid sequence having at least 98% sequence identity to SEQ ID NO:2. 
     
     
         73 . The renalase protein of  claim 70 , wherein the renalase protein comprises the amino acid sequence of SEQ ID NO:2. 
     
     
         74 . The renalase protein of  claim 70 , wherein the renalase protein comprises an amino acid sequence encoded by SEQ ID NO:1 or SEQ ID NO:3. 
     
     
         75 . The renalase protein of  claim 70 , wherein the catecholamine is dopamine, epinephrine, or norepinephrine. 
     
     
         76 . The renalase protein of  claim 70 , wherein the host cell is a bacterial host cell. 
     
     
         77 . The renalase protein of  claim 70 , wherein the host cell is a yeast cell. 
     
     
         78 . The renalase protein of  claim 70 , wherein the host cell is an insect or vertebrate host cell. 
     
     
         79 . The renalase protein of  claim 70 , wherein the renalase protein is produced in the presence of exogenous FAD added at the time of inducing protein synthesis. 
     
     
         80 . The renalase protein of  claim 70 , wherein the renalase protein comprises a purification tag. 
     
     
         81 . The renalase protein of  claim 70 , wherein the renalase protein is produced by denaturing and refolding. 
     
     
         82 . The renalase protein of  claim 81 , wherein the renalase protein is produced by recovering from inclusion bodies, denaturing with urea, and refolding. 
     
     
         83 . A pharmaceutical composition comprising the renalase protein of  claim 70  and a pharmaceutically acceptable carrier, diluent, or vehicle. 
     
     
         84 . The pharmaceutical composition of  claim 83 , formulated for injectable administration. 
     
     
         85 . A method for identifying a subject in need of renalase therapy, comprising:
 determining the level of circulating renalase protein in the subject, wherein a subject having lower levels of renalase protein as compared to a normal level is in need of renalase therapy.   
     
     
         86 . The method of  claim 85 , wherein the subject has a cardiovascular disease or renal disease. 
     
     
         87 . The method of  claim 85 , wherein the level of renalase protein is determined using an antibody specific for renalase.