Producing method of synthetic collagen nano-fiber

Abstract

With respect to synthetic collagen that has so far been difficult to be nano-fiberized, a method for producing uniform and long fibrous nano-fibers containing a synthetic collagen is described. The nano-fibers contain a polypeptide having a peptide fragment represented by Formula ( 1 ): -(Pro-Y-Gly) n   (1), wherein Y represents hydroxyproline or proline, and n is an integar ranging from 5 to 9000. The producing method includes a step of preparing a spinning solution containing the polypeptide and a polymer, and a step of spinning with an electrospinning method using the spinning solution.

What is claimed is: 
     
         1 . A method of producing a nano-fiber that contains a polypeptide having a peptide fragment represented by Formula (1):
   -(Pro-Y-Gly) n -   (1),
   
       wherein Y represents a hydroxyproline or proline, and n is an integer ranging from  5  to 9000, the producing method comprising: preparing a spinning solution containing the polypeptide and a polymer, and spinning with an electrospinning method using the spinning solution. 
     
     
         2 . The method according to  claim 1 , wherein a concentration of the polypeptide in the spinning solution is from 0.1 wt % to 10 wt %. 
     
     
         3 . The method according to  claim 1 , wherein a concentration of the polymer in the spinning solution is from 0.1 wt % to 10 wt %. 
     
     
         4 . The method according to  claim 1 , wherein a weight ratio of the polypeptide to the polymer in the spinning solution is from 10:1 to 1:40. 
     
     
         5 . The method according to  claim 1 , wherein the polymer comprises one, two or more selected from the group consisting of natural collagen, polyethylene glycols, polyvinyl alcohols, and polyglycolic acids. 
     
     
         6 . A nano-fiber, containing a polymer and a peptide fragment represented by Formula (1):
   -(Pro-Y-Gly) n -   (1),
   
       wherein Y represents hydroxyproline or proline, and n is an integer ranging from 5 to 9000.