Method of making a paired tag library for nucleic acid sequencing

Abstract

The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

1 - 91 . (canceled) 
     
     
         92 . A plurality of circular nucleic acid molecules, the individual circular molecules in the plurality comprising:
 (a) a universal sequence region common to the plurality of circular nucleic acid molecules and having two nicks; and   (b) a target sequence region that differs between different molecules of the plurality of circular molecules.   
     
     
         93 . The molecules of  claim 92 , wherein the universal sequence region comprises a double-stranded nucleic acid. 
     
     
         94 . The molecules of  claim 92 , wherein the universal sequence further comprises a binding moiety. 
     
     
         95 . The molecule of  claim 94 , wherein the binding moiety comprises biotin. 
     
     
         96 . The molecules of  claim 94 , further comprising a purifying moiety. 
     
     
         97 . The molecules of  claim 96  wherein the purifying moiety comprises streptavidin. 
     
     
         98 . The molecule of  claim 92 , further comprising a nick translating enzyme. 
     
     
         99 . The molecule of  claim 98 , wherein the nick translating enzyme comprises DNA polymerase I. 
     
     
         100 . A plurality of circular nucleic acid molecules, the individual circular molecules in the plurality comprising:
 (a) a universal sequence region common to the plurality of circular nucleic acid molecules, the universal sequence region having a first end and a second end;   (b) a target sequence region that differs between different molecules of the plurality of circular molecules, the target sequence region having a first end and a second end;   (c) a first nick between the first end of the universal sequence region and the first end of the target sequence region; and   (d) a second nick between the second end of the universal sequence region and the second end of the target sequence region.