Method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves

Abstract

A supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves, which involves the following steps: 1) cutting the tobacco leaves into shreds and placing the shreds into an extraction vessel with supercritical CO 2 fluid; 2) setting the extraction pressure and extraction temperature of supercritical CO 2 fluid about 100 to 350 bar and 35 to 70° C., extracting statically the tobacco shreds for 10 to 35 minutes in the condition, then setting the dynamic extracting speed of the supercritical CO 2 fluid about 1.0 to 3.5 L/min, and extracting dynamically the tobacco shreds for 5 to 15 minutes; 3) collecting the obtained supercritical CO 2 fluid extract, and obtaining the key fragrant substances, which act as the determinants for tobacco fragrance, after completely volatilizing CO 2 .

1 . A method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves, comprising:
 1) pretreatment of tobacco leaves: shredding the tobacco leaves and placing the tobacco shreds into an extraction vessel with supercritical CO 2  fluid;   2) setting the extraction pressure and the extraction temperature of the supercritical CO 2  fluid to 100 to 350 bar and 35° C. to 70° C., performing static extraction on the tobacco shreds for 10 to 35 min under the condition, then setting the dynamic extraction flow rate of the supercritical CO 2  fluid to 1.0 to 3.5 L/min, and performing dynamic extraction on the tobacco shreds for 5 to 15 min; and   3) collecting the obtained supercritical CO 2  fluid extract, and obtaining key fragrant substances that act as determinants after complete evaporation of CO 2 .   
     
     
         2 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to  claim 1 , wherein the used CO 2  is food grade CO 2 . 
     
     
         3 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to  claim 1 , wherein in Step 1), the width of the tobacco shreds is 0.8 mm to 1.2 mm. 
     
     
         4 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to  claim 1 , wherein in Step 2), the extraction pressure and the extraction temperature of the supercritical CO 2  fluid are set to 250 to 320 bar and 45 to 55° C. 
     
     
         5 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to  claim 1 , wherein in Step 2), static extraction is performed on the tobacco shreds for 20 to 30 min. 
     
     
         6 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to  claim 1 , wherein in Step 2), the dynamic extraction flow rate of the supercritical CO 2  fluid is set to 2.0 to 3.5 L/min. 
     
     
         7 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to  claim 1 , wherein in Step 2), dynamic extraction is performed on the tobacco shreds for 10 to 15 min. 
     
     
         8 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 1 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance. 
     
     
         9 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 2 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance. 
     
     
         10 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 3 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance. 
     
     
         11 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 4 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance. 
     
     
         12 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 5 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance. 
     
     
         13 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 6 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance. 
     
     
         14 . The method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves according to any one of  claim 7 , wherein the tobacco leaves comprise three types of typical fragrance tobacco leaves and intermediary fragrance tobacco leaves, the three typical fragrances are strong fragrance, mild fragrance, and intermediate fragrance, the intermediary fragrances comprise strong-to-intermediate fragrance, intermediate-to-strong fragrance, mild-to-intermediate fragrance, intermediate-to-mild fragrance, mild-over-strong fragrance, and strong-over-mild fragrance.