Agent for reducing the useable calorie content of food and for therapeutic reduction of weight, in particular for use in the case of adiposity (obesity)

Abstract

There are disclosed compositions and methods for treating obesity using 5-D-fructose dehydrogenase. Other embodiments are also described.

1 - 262 . (canceled) 
     
     
         263 . A mammalian ingestible composition which is adapted for oral administration selected from a pharmaceutical composition and a dietary supplement, said composition being in unit dosage form, said composition comprising 5-D-fructose dehydrogenase and a carrier or excipient that is acceptable for use in pharmaceutical compositions or foodstuffs, said composition further comprising a second enzyme selected from the group consisting of a glucose isomerase, maltase, invertase, lactase, alpha-amylase, beta-amylase, glucoamylase, pullulanase, isoamylase, amyloglucosidase, cyclomaltodextrin glucanotransferase, and mixtures thereof. 
     
     
         264 - 266 . (canceled) 
     
     
         267 . The composition according to  claim 263  which is in a form selected from (a) the group consisting of a tablet, capsule, gel cap, pellet and dragee, (b) powder or (c) liquid form as solution, drops, suspension or gel. 
     
     
         268 . The composition according to  claim 263  wherein said 5-D-fructose dehydrogenase is protected by a coating which is stable at pH below 4. 
     
     
         269 . The composition of according to  claim 268  wherein said coating protects the entire dosage unit. 
     
     
         270 . The composition according to  claim 263  wherein said 5-D-fructose dehydrogenase is microencapsulated. 
     
     
         271 . The composition according to  claim 263  wherein said 5-D-fructose dehydrogenase constitutes between 5 and 99.9% by weight of the composition. 
     
     
         272 . The composition according to  claim 263  wherein said unit dosage contains between 50 and 1 million units of 5-D-fructose dehydrogenase activity. 
     
     
         273 . The composition according to  claim 263  wherein said composition comprises a coating which dissolves at a pH of 5.5 or higher. 
     
     
         274 . The composition according to  claim 263  wherein said 5-D-fructose dehydrogenase is not contained in an inorganic-based sol-gel biocompatible matrix. 
     
     
         275 . The composition according to  claim 263  wherein said second enzyme is a glucose isomerase. 
     
     
         276 . The composition according to  claim 275 , further comprising a third enzyme selected from the group consisting of invertase, maltase, lactase, alpha-amylase, beta-amylase, glucoamylase, pullulanase, isoamylase, amyloglucosidase, cyclomaltodextrin glucanotransferase and mixtures thereof. 
     
     
         277 . The composition according to  claim 263 , wherein said second enzyme is selected from the group consisting of lactase, alpha-amylase, beta-amylase, glucoamylase, pullulanase, isoamylase, amyloglucosidase, cyclomaltodextrin glucanotransferase and mixtures thereof. 
     
     
         278 . A foodstuff which comprises 5-D-fructose dehydrogenase wherein at least some of said 5-D-fructose dehydrogenase is present in said foodstuff in a form in which said 5-D-fructose dehydrogenase will be released in active form to act on fructose which is present in or released from said foodstuff after ingestion of said foodstuff. 
     
     
         279 . A foodstuff according to  claim 278  which further comprises a second enzyme selected from the group consisting of a glucose isomerase, invertase, maltase, lactase, alpha-amylase, beta-amylase, glucoamylase, pullulanase, isoamylase, amyloglucosidase, cyclomaltodextrin glucanotransferase and mixtures thereof,
 wherein at least one of the following is true: (a) at least some of said second enzyme is present in said foodstuff in active form in which said second enzyme can act on a substrate which is present in or released from said foodstuff before ingestion of said foodstuff, and (b) at least some of said second enzyme is present in said foodstuff in a form in which said second enzyme will be released in active form to act on a substrate which is present in or released from said foodstuff after ingestion of said foodstuff. 
 
     
     
         280 - 281 . (canceled) 
     
     
         282 . A foodstuff according to  claim 279 , wherein said foodstuff is selected from the group consisting of a baked foodstuff, a cooked foodstuff, and a liquid foodstuff. 
     
     
         283 . A foodstuff according to  claim 279  wherein said 5-D-fructose dehydrogenase is not contained in an inorganic-based sol-gel biocompatible matrix. 
     
     
         284 . A foodstuff according to  claim 279  wherein said second enzyme is a glucose isomerase. 
     
     
         285 . A foodstuff according to  claim 284  wherein at least one of said 5-D-fructose dehydrogenase and said glucose isomerase is microencapsulated. 
     
     
         286 . (canceled) 
     
     
         287 . A method of treating adiposity, reducing the effect of adiposity on a mammalian body, reducing the effect of D-fructose in a mammalian body, or reducing the effect of total fructose in a mammalian body, comprising administering to a subject in need of such treatment or reduction an efficacious amount of 5-D-fructose dehydrogenase. 
     
     
         288 . A method according to  claim 287 , further comprising administering to said subject a second enzyme selected from the group consisting of a glucose isomerase, invertase, maltase, lactase, alpha-amylase, beta-amylase, glucoamylase, pullulanase, isoamylase, amyloglucosidase, cyclomaltodextrin glucanotransferase and mixtures thereof. 
     
     
         289 - 310 . (canceled)