Indoleamine 2,3-Dioxygenase-2

Abstract

The nucleotide and amino acid sequences of indoleamine 2,3-dioxygenase-2 (IDO2) and methods of use thereof are provided.

1 . An isolated indoleamine 2,3-dioxygenase-2 (IDO2) protein comprising an amino acid sequence which has at least 80% identity with SEQ ID NO: 1. 
     
     
         2 . (canceled) 
     
     
         3 . The isolated IDO2 protein of  claim 1 , where said amino acid sequence is SEQ ID NO: 1. 
     
     
         4 . (canceled) 
     
     
         5 . (canceled) 
     
     
         6 . A method for identifying a compound which modulates IDO2 activity comprising:
 a) contacting the IDO2 protein of  claim 1  with a test compound; and   b) determining tryptophan catabolism activity of the IDO2 protein;   
       wherein a change in the tryptophan catabolism activity in the presence of the test compound compared to the tryptophan catabolism activity in the absence of the test compound indicates that the test compound can modulate IDO2 activity. 
     
     
         7 . The method of  claim 6 , wherein said IDO2 protein of step a) is expressed in a cell. 
     
     
         8 . The method of  claim 6 , wherein IDO2 activity is assessed by measuring liver inhibitory protein (LIP). 
     
     
         9 . (canceled) 
     
     
         10 . An isolated splice variant of the IDO2 protein of  claim 1 . 
     
     
         11 . An isolated nucleic acid molecule encoding the IDO2 splice variant of  claim 10 . 
     
     
         12 . A transgenic mouse harboring homozygous null mutations in its endogenous IDO2 gene. 
     
     
         13 . The isolated IDO2 protein of  claim 1 , wherein said amino acid sequence has at least 85% identity with SEQ ID NO: 1. 
     
     
         14 . The isolated IDO2 protein of  claim 1 , wherein said amino acid sequence has at least 90% identity with SEQ ID NO: 1. 
     
     
         15 . The isolated IDO2 protein of  claim 1 , wherein said amino acid sequence has at least 95% identity with SEQ ID NO: 1. 
     
     
         16 . The isolated IDO2 protein of  claim 1 , wherein said amino acid sequence has at least 97% identity with SEQ ID NO: 1. 
     
     
         17 . The isolated IDO2 protein of  claim 1 , wherein said amino acid sequence has at least 99% identity with SEQ ID NO: 1.