Wise/sost nucleic acid sequences and amino acid sequences

Abstract

The present invention relates to nucleic acid sequences and amino acid sequences which influence bone deposition, the Wnt pathway, ocular development, tooth development, and may bind to LRP. The nucleic acid sequence and polypeptides include Wise and Sost as well as a family of molecules which express a cysteine knot polypeptide. Additionally, the present invention relates to various molecular tools derived from the nucleic acids and polypeptides including vectors, transfected host cells, monochronal antibodies, Fab fragments, and methods for impacting the pathways.

1 - 235 . (canceled) 
     
     
         236 . A method for preventing a Sost polypeptide from binding to an LRP selected from the group consisting of LRP5 and LRP6 comprising:
 (a) isolating a Sost nucleic acid sequence selected from a group consisting of SEQ ID No. 6-7, 9-11, 13-14, 101-102, 110, and 111;   (b) forming a plasmid vector with a Sost nucleic acid sequence from (a) and transfecting a host cell with the plasmid vector whereby the host cell expresses the Sost nucleic acid sequence to produce a Sost polypeptide;   (c) immunizing a host organism with the Sost polypeptide;   (d) isolating an antibody to the Sost polypeptide from the host;   (e) contacting the antibody with the Sost polypeptide that is encoded by the Sost nucleic acid, wherein the antibody prevents the Sost polypeptide from binding to an LRP selected from LRP5 and LRP6.   
     
     
         237 . The method according to  claim 236 , wherein the antibody is monoclonal. 
     
     
         238 . The method according to  claim 236 , wherein the antibody is polyclonal. 
     
     
         239 . The method according to  claim 236 , wherein the antibody is recombinant. 
     
     
         240 . The method according to  claim 236 , wherein the antibody was treated to produce an antibody fragment prior to contacting with the Sost polypeptide in step (e). 
     
     
         241 . The method according to  claim 240 , wherein the antibody fragment is selected from the group consisting of a Fab, a F(ab) 2 , a Fv, and an antibody combining region. 
     
     
         242 . The method according to  claim 236 , wherein the LRP is selected from the group consisting of SEQ ID Nos. 80-84. 
     
     
         243 . The method according to  claim 242 , wherein the antibody is monoclonal. 
     
     
         244 . The method according to  claim 242 , wherein the antibody is polyclonal. 
     
     
         245 . The method according to  claim 242 , wherein the antibody is recombinant. 
     
     
         246 . The method according to  242 , wherein the antibody was treated to produce an antibody fragment prior to contacting with the Sost polypeptide in step (e). 
     
     
         247 . The method according to  claim 246 , wherein the antibody fragment is selected from the group consisting of a Fab, a F(ab) 2 , a Fv, and an antibody combining region.