Use of Molecular Chaperones for the Enhanced Production of Secreted, Recombinant Proteins in Mammalian Cells

Abstract

The present invention relates to a method for increased production of a secreted, recombinant protein product through the introduction of molecular chaperones in a mammalian host cell. The present invention also relates to a mammalian host cell with enhanced expression of a secreted recombinant protein product by coexpressing at least one chaperone protein.

1 . A mammalian host cell for enhanced expression of a recombinant protein product, said mammalian cell having genetic material coding for expression of said recombinant protein product and transformed with at least one expression vector comprising DNA encoding calnexin as a chaperone protein. 
     
     
         2 - 71 . (canceled) 
     
     
         72 . The mammalian host cell according to  claim 1 , wherein the recombinant protein product is secreted. 
     
     
         73 . The mammalian host cell according to  claim 72 , wherein the genetic material coding for the expression of said recombinant protein product is integrated into host cell DNA. 
     
     
         74 . The mammalian host cell according to  claim 73 , further transformed with an expression vector comprising DNA enclosing a glutamine synthetase protein. 
     
     
         75 . The mammalian host cell according to  claim 73 , wherein said recombinant protein is bikunin or fragment thereof. 
     
     
         76 . The mammalian host cell according to  claim 73 , wherein said recombinant protein is Factor VIII or fragment thereof.