Tumor Suppressor Gene, p28ING5

Abstract

This disclosure provides a novel tumor suppressor, referred to as p28ING5, nucleic acid molecules encoding this protein, and methods of making and using these molecules. Also provided are methods of ameliorating, treating, detecting, prognosing, and diagnosing diseases and conditions associated with abnormal p28ING5 expression, such as neoplasia. Kits are also provided.

We claim: 
     
         1 . A method of inhibiting cellular proliferation, comprising:
 transfecting a cell with an expression vector comprising a promoter operably linked to nucleotides 1-39 and 723-1068 of the sequence set forth in SEQ ID NO: 1, thereby inhibiting cellular proliferation.   
     
     
         2 . The method of  claim 1 , wherein the cell is a tumor cell. 
     
     
         3 . The method of  claim 1 , wherein the expression vector comprises a promoter operably linked to a nucleic acid sequence as set forth in SEQ ID NO: 1, or a conservative substitution thereof. 
     
     
         4 . The method of  claim 1 , wherein the expression vector comprises a promoter sequence operably linked to the p281NG5 recombinant nucleotide consisting of the nucleotide sequence of SEQ ID NO: 1. 
     
     
         5 . The method of  claim 1 , wherein the cell is a colonic cancer cell.