Fertility restorer gene and fertility restoration method for cw-type male sterile cytoplasm of rice

Abstract

Mainly provided is a technique for directly identifying the genotype at locus Rf17 based on the specific base sequence data thereof. Also provided is a technique for artificially constructing a fertility-restored line. A method of restoring the fertility of CW-type cytoplasmic male sterile rice by inhibiting or reducing the expression of a gene comprising the base sequence represented by SEQ ID NO:2 in the above-described rice, and a method for determining the presence or absence of gene Rf17, which is a fertility restorer gene for CW-type cytoplasmic male sterility, comprising identifying a single nucleotide polymorphism (SNP) in the base at the 1812 position of the base sequence represented by SEQ ID NO:1 in the rice to be examined.

1 - 14 . (canceled) 
     
     
         15 . A polynucleotide consisting of a base sequence selected from the group consisting of:
 (a) a base sequence at least 1611-4835 positions of the base sequence represented by SEQ ID NO:1;   (b) a base sequence that hybridizes with the base sequence (a) or its complementary sequence under stringent conditions, and   (c) a base sequence having homology of about 95% or more with the base sequence (a).   
     
     
         16 . A vector comprising the polynucleotide according to  claim 15   
     
     
         17 . A plant that is transformed with the vector according to  claim 16 . 
     
     
         18 . The plant according to  claim 17 , said plant is transformed by means of  Agrobacterium  method. 
     
     
         19 . A plant of CW-type cytoplasmic male sterile rice, said plant is transformed with the vector according to  claim 16 , so that its fertility has been restored. 
     
     
         20 . The plant according to  claim 19 , said plant is transformed by means of  Agrobacterium  method. 
     
     
         21 . A polynucleotide consisting of contiguous 10-30 bp comprising a base “T” at position 1812 of the base sequence represented by SEQ ID NO:1. 
     
     
         22 . A primer, marker or probe comprising the polynucleotide according to  claim 21  for use in the identification of a single nucleotide polymorphism in a base at 1812 position of the base sequence represented by SEQ NO:1.