Methods for extracting platelets and compositions obtained therefrom

Abstract

This invention provides methods for extracting platelets, compositions obtained therefore, and methods for using the same.

What is claimed is: 
     
         1 . A composition comprising a growth factor produced by a process comprising:
 disrupting pelletized platelets in an extraction solution to produce an insoluble material portion and the extraction solution comprising a growth factor; and   removing insoluble materials and proteins having molecular weight of 2 kD or less from said extraction solution to produce a composition comprising a growth factor.   
     
     
         2 . The composition of  claim 1 , wherein said method for disrupting pelletized platelets comprises sonication, homogenization, nitrogen cavitation, a French pressure cell, or a combination thereof. 
     
     
         3 . The composition of  claim 1 , wherein said step of removing insoluble materials and proteins comprises:
 (i) filtering said extraction solution using an ultrafiltration process to remove insoluble materials and proteins having molecular weight of 2 kD or less; or   (ii) removing insoluble materials from said extraction solution; and
 (a) dialyzing said extraction solution against an acidic solution using a semipermeable membrane having 2 kD or less MWCO; or 
 (b) filtering said extraction solution using an ultrafiltration process to remove proteins having molecular weight of 2 kD or less. 
   
     
     
         4 . The composition of  claim 3 , wherein said step of dialyzing comprises using a semipermeable membrane having 8 kD or less MWCO. 
     
     
         5 . The composition of  claim 1 , wherein said process further comprises subjecting said extraction solution to an ultrafiltration process to remove any microorganisms that maybe present. 
     
     
         6 . The composition of  claim 5 , wherein said process further comprises lyophilizing said extraction solution to produce a solid composition comprising the growth factor. 
     
     
         7 . The composition of  claim 6 , wherein said process further comprises dissolving said solid composition in a solution to produce a fluid composition comprising the growth factor. 
     
     
         8 . The composition of  claim 1 , wherein said growth factor comprises VEGF, TGF-β 1 , TGF-β 2 , PDGF-BB, EGF, or a mixture thereof. 
     
     
         9 . A wound healing composition comprising therapeutically effective amount of a composition of  claim 1  and a pharmaceutically acceptable carrier. 
     
     
         10 . The wound healing composition of  claim 9 , wherein said pharmaceutically acceptable carrier comprises a bandage. 
     
     
         11 . The wound healing composition of  claim 9 , wherein the pharmaceutically acceptable carrier comprises a pharmaceutically acceptable polymer. 
     
     
         12 . A method for regenerating a tissue or treating a wound comprising contacting a desired site for tissue regeneration or the wound with an angiogenic composition comprising a composition of  claim 1 . 
     
     
         13 . The method of  claim 12 , wherein the desired site for tissue regeneration comprises a burn site, a graft donor site, an oral and maxillofacial surgery site, a mucogingival surgery site, a perioplastic surgery site, an oral mucosa site, or a combination thereof. 
     
     
         14 . A method for reducing the risk of microbial infection in a wound comprising treating the wound with a wound treatment composition comprising a composition of  claim 1 .