Glyphosate resistant 5-enolpyruvylshikimate-3-phosphate (epsp) synthase

Abstract

A plant comprising SEQ. ID. NO. 2 or a functional portion thereof, wherein SEQ ID NO. 2 is not native to said plant. A glyphosate resistant grass of economic value comprises a nucleic acid molecule that encodes a EPSPS enzyme. In some embodiments, the nucleic acid molecule comprises a sequence of SEQ. ID. NO. 1, or a functional portion thereof. In some embodiments, the EPSPS enzyme can be a polypeptide molecule comprising an amino acid sequence that is essentially of SEQ. ID. NO. 2, or portion thereof. Embodiments include a DNA construct comprising a SEQ. ID. NO. 1 or a functional portion thereof and transgenic methods for inserting the DNA construct into a plant. Some embodiments include non-transgenic glyphosate resistant turfgrasses.

1 - 73 . (canceled) 
     
     
         74 . An isolated glyphosate resistant gene obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007. 
     
     
         75 . The isolated glyphosate resistant gene according to  claim 74 , wherein the gene comprises a DNA sequence at least 93% homologous to SEQ ID NO:1 that encodes a functional glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). 
     
     
         76 . The isolated glyphosate resistant gene according to  claim 75 , wherein the gene comprises a DNA sequence 100% identical to SEQ ID NO:1. 
     
     
         77 . An isolated nucleic acid molecule obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007 comprising SEQ ID NO:1 or a functional portion thereof. 
     
     
         78 . The isolated nucleic acid molecule according to  claim 77 , wherein the nucleic acid molecule encodes a glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) having an amino acid sequence at least 80% homologous to SEQ ID NO:2. 
     
     
         79 . The isolated nucleic acid molecule according to  claim 78 , wherein the nucleic acid molecule encodes a glyphosate-resistant EPSPS having an amino acid sequence at least 90% homologous to SEQ ID NO:2. 
     
     
         80 . The isolated nucleic acid molecule according to  claim 77 , wherein the nucleic acid molecule encodes a glyphosate-resistant EPSPS having an amino acid sequence 100% homologous to SEQ ID NO:2. 
     
     
         81 . An isolated DNA construct comprising the isolated nucleic acid molecule according to  claim 77 . 
     
     
         82 . A DNA construct comprising a polynucleotide segment that has a base sequence of SEQ ID NO:1, or a functional portion thereof, and that encodes SEQ ID NO:2, the polynucleotide segment being operably attached to expression regulatory elements that can be used by a host cell to express a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide encoded by the polynucleotide segment. 
     
     
         83 . A recombinant DNA molecule comprising a structural DNA segment that encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide comprising sequence of SEQ ID NO:2, or a functional portion thereof, operably linked to expression regulatory elements that can be used by a host cell to express the EPSPS polypeptide or peptide encoded by the structural DNA segment. 
     
     
         84 . The recombinant DNA molecule according to  claim 83 , wherein the expression regulatory elements are those that can be used by a plant cell to achieve expression of the structural DNA sequence. 
     
     
         85 . The recombinant DNA molecule according to  claim 83 , wherein the expression regulatory elements include a plant promoter that is heterologous to the structural DNA sequence. 
     
     
         86 . The recombinant DNA molecule according to  claim 83  further comprising a DNA sequence that is operably linked to the structural DNA segment and that encodes an amino-terminal chloroplast transit peptide. 
     
     
         87 . The recombinant DNA molecule according to  claim 83 , wherein structural DNA segment that encodes the EPSPS polypeptide has a nucleic acid sequence at least 93% identical to SEQ ID NO:1. 
     
     
         88 . An isolated glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoded by a DNA molecule comprising SEQ ID NO:1 or a functional portion thereof. 
     
     
         89 . An isolated glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) comprising the amino acid sequence of SEQ ID NO:2, or a functional portion SEQ ID NO:2, obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007. 
     
     
         90 . An isolated glyphosate resistant protein (GRP) encoded by a DNA molecule obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007.