Heat-Stable Persephonella Carbonic Anhydrases and Their Use

Abstract

The present invention relates to use of Persephonella carbonic anhydrase in CO 2 extraction, e.g., from flue gas, natural gas, biogas or ambient air. The Persephonella carbonic anhydrases are especially well suited for these purpose due to their extreme thermostability.

1 - 32 . (canceled) 
     
     
         33 . A method for extracting carbon dioxide comprising treating a carbon dioxide-containing medium with a carbonic anhydrase obtainable from or producible by a strain belonging to the genus  Persephonella.    
     
     
         34 . A composition comprising an excipient and a carbonic anhydrase selected from the group consisting of
 a) a polypeptide derived from or producible by  Persephonella marina  DSM 14350; or   b) a polypeptide having an amino acid sequence corresponding to amino acid residues 20 to 243 of SEQ ID NO: 2 or amino acid residues 29 to 251 of SEQ ID NO: 4; or   c) a polypeptide which is at least 60% identical to amino acid residues 20 to 243 of SEQ ID NO: 2 or amino acid residues 29 to 251 of SEQ ID NO: 4; or   d) a fragment of (a), (b) or (c) having carbonic anhydrase activity; or   e) a polypeptide encoded by a nucleic acid sequence which hybridizes under medium stringency conditions with:
 i) a polynucleotide sequence encoding a mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4; or 
 ii) a polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3; or 
 iii) a subsequence of (i) or (ii), of at least 100 contiguous nucleotides, or 
 iv) a complementary strand of (i) or (ii); or 
   f) a polypeptide encoded by a nucleic acid sequence which, because of the degeneracy of the genetic code, does not hybridize with the polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3, but which codes for a polypeptide having an amino acid sequence according to b) or c); or   g) a polypeptide encoded by a nucleic acid sequence which is at least 60% identical to SEQ ID NO: 1 or SEQ ID NO: 3.   
     
     
         35 . The composition of  claim 34 , where the composition comprises a matrix suitable for immobilization. 
     
     
         36 . The composition of  claim 35 , where that matrix is selected from the group consisting of beads, fabrics, fibers, hollow fibers, membranes, particulates, porous surfaces, rods, and tubes. 
     
     
         37 . A bioreactor for extracting carbon dioxide, where said reactor comprises a carbonic anhydrase obtainable from or producible by a strain belonging to the genus  Persephonella.    
     
     
         38 . The bioreactor of  claim 37 , where said reactor comprises a carbonic anhydrase obtainable from a strain selected from the group of species consisting of  Persephonella marina, Persephonella hydrogeniphila  or  Persephonella guaymasensis.    
     
     
         39 . The bioreactor of  claim 37 , where said reactor comprises a carbonic anhydrase obtainable from a strain deposited as DSM 14350, DSM 15105 or DSM 14351. 
     
     
         40 . The bioreactor of  claim 37 , wherein the carbonic anhydrase is
 a) derived from or producible by  Persephonella marina  DSM 14350; or   b) a polypeptide having an amino acid sequence corresponding to amino acid residues 20 to 243 of SEQ ID NO: 2 or amino acid residues 29 to 251 of SEQ ID NO: 4; or   c) a polypeptide which is at least 60% identical to amino acid residues 20 to 243 of SEQ ID NO: 2 or amino acid residues 29 to 251 of SEQ ID NO: 4; or   d) a fragment of (a), (b) or (c) having carbonic anhydrase activity; or   e) a polypeptide encoded by a nucleic acid sequence which hybridizes under medium stringency conditions with:
 i) a polynucleotide sequence encoding a mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4; or 
 ii) a polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3; or 
 iii) a subsequence of (i) or (ii), of at least 100 contiguous nucleotides, or 
 iv) a complementary strand of (i) or (ii); or 
   f) a polypeptide encoded by a nucleic acid sequence which, because of the degeneracy of the genetic code, does not hybridize with the polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3, but which codes for a polypeptide having an amino acid sequence according to b) or c); or   g) a polypeptide encoded by a nucleic acid sequence which is at least 60% identical to SEQ ID NO: 1 or SEQ ID NO: 3.   
     
     
         41 . The bioreactor of  claim 37 , where said reactor comprises the following elements:
 a) at least one absorption module;   b) at least one precipitation stage;   c) a carrier liquid; and   d) means for connecting the absorption module(s) to the precipitation stage(s) such that the carrier liquid can be passed from the absorption module(s) to the precipitation stage(s).   
     
     
         42 . The bioreactor of  claim 37 , where said reactor comprises the following elements:
 a) at least one absorption module;   b) at least one desorption module;   c) a carrier liquid; and   d) means for connecting the absorption module(s) and the desorption module(s) such that the carrier liquid can be passed from the absorption module(s) to the desorption module(s).   
     
     
         43 . The bioreactor of  claim 37 , wherein the carbonic anhydrase is entrapped in the absorption module or in the desorption module or in both modules, or in the precipitation stage. 
     
     
         44 . The bioreactor of  claim 37  where the reactor further comprises a bicarbonate-based carrier liquid. 
     
     
         45 . The bioreactor of  claim 37 , where the reactor further comprises an amine-based carrier liquid. 
     
     
         46 . The bioreactor of  claim 45 , where the amine-based carrier liquid includes Tris or MDEA. 
     
     
         47 . The bioreactor of  claim 44 , where the reactor further comprises HPO 4   2- , such as K 2 HPO 4  or Na 2 HPO 4 .