US2013130391A1PendingUtilityA1

Method of analyzing hemoglobins

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Assignee: TAIRA HIROAKIPriority: Mar 31, 2010Filed: Mar 22, 2011Published: May 23, 2013
Est. expiryMar 31, 2030(~3.7 yrs left)· nominal 20-yr term from priority
Inventors:Hiroaki TairaTakayuki Oka
G01N 33/721G01N 30/06G01N 2030/8831G01N 2030/067B01D 15/362G01N 30/34G01N 30/96G01N 2030/8822G01N 2440/00
52
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Claims

Abstract

An object of the present invention is to provide a method for analyzing hemoglobins which can accurately separate hemoglobins in a short time by liquid chromatography. The present invention provides a method for analyzing hemoglobins by liquid chromatography which includes pre-treating a sample with an oxidant and a binder for trivalent heme iron.

Claims

exact text as granted — not AI-modified
1 . A method for analyzing hemoglobins by liquid chromatography, comprising pre-treating a sample with an oxidant and a binder for trivalent heme iron. 
     
     
         2 . The method for analyzing hemoglobins according to  claim 1 ,
 wherein the oxidant is a substance capable of transforming hemoglobins to their met-forms.   
     
     
         3 . The method for analyzing hemoglobins according to  claim 2 ,
 wherein the oxidant is a nitrite salt.   
     
     
         4 . The method for analyzing hemoglobins according to  claim 3 ,
 wherein the oxidant is sodium nitrite.   
     
     
         5 . The method for analyzing hemoglobins according to  claim 1 ,
 wherein the binder for trivalent heme iron is a substance that binds to trivalent iron in methemoglobin.   
     
     
         6 . The method for analyzing hemoglobins according to  claim 5 ,
 wherein the binder for trivalent heme iron is an azide.   
     
     
         7 . The method for analyzing hemoglobins according to  claim 6 ,
 wherein the binder for trivalent heme iron is sodium azide.   
     
     
         8 . The method for analyzing hemoglobins according to  claim 1 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.   
     
     
         9 . The method for analyzing hemoglobins according to  claim 2 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.   
     
     
         10 . The method for analyzing hemoglobins according to  claim 3 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.   
     
     
         11 . The method for analyzing hemoglobins according to  claim 4 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.   
     
     
         12 . The method for analyzing hemoglobins according to  claim 5 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.   
     
     
         13 . The method for analyzing hemoglobins according to  claim 6 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.   
     
     
         14 . The method for analyzing hemoglobins according to  claim 7 ,
 wherein the liquid chromatography is cation-exchange liquid chromatography.

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