Method for producing oil containing polyunsaturated fatty acid using lipase

Abstract

A method for lowering saturated fatty acid content, the method comprising concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25° C.

1 . A method for lowering saturated fatty acid content, the method comprising the step of concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25° C. 
     
     
         2 . The method according to  claim 1 ; wherein the lipase is derived from a microorganism selected from among a group consisting of microorganisms belonging to the genera  Candida, Alcaligenes, Burkholderia, Pseudomonas, Thermomyces , and  Rhizomucor.    
     
     
         3 . A glyceride containing a polyunsaturated fatty acid, of which saturated fatty acid fraction is not more than 12 area percent, and which is produced by a method comprising the step of concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25° C. 
     
     
         4 . The glyceride containing the polyunsaturated fatty acid according to  claim 3 ; wherein the lipase is derived from a microorganism selected from among a group consisting of microorganisms belonging to the genera  Candida, Alcaligenes, Burkholderia, Pseudomonas, Thermomyces , and  Rhizomucor.    
     
     
         5 . A glyceride produced by concentrating a polyunsaturated fatty acid by lipase reaction; wherein content of docosahexaenoic acid in the glyceride is not less than 40 area percent; and content of saturated fatty acids in the glyceride is not more than 12 area percent. 
     
     
         6 . The glyceride according to  claim 5 ; wherein content of docosahexaenoic acid in the glyceride is not less than 46 area percent. 
     
     
         7 . The glyceride according to  claim 5 ; wherein content of saturated fatty acids is not more than 10 area percent. 
     
     
         8 . The glyceride according to  claim 5 ; wherein content of palmitic acid is not more than 8 area percent. 
     
     
         9 . The glyceride according to  claim 8 ; wherein content of palmitic acid is not more than 6 area percent. 
     
     
         10 . The glyceride according to  claim 5 ; wherein the fraction of triglyceride in the glyceride is not less than 80 area percent. 
     
     
         11 . The glyceride according to  claim 10 ; wherein the fraction of triglyceride in the glyceride is not less than 85 area percent. 
     
     
         12 . A glyceride having polyunsaturated fatty acid concentrated by lipase reaction; wherein content of eicosapentaenoic acid in the glyceride is not less than 25 area percent; and content of saturated fatty acids is not more than 20 area percent. 
     
     
         13 . The glyceride according to  claim 12 ; wherein content of eicosapentaenoic acid in the glyceride is not less than 28 area percent. 
     
     
         14 . The glyceride according to  claim 12 ; wherein content of the saturated fatty acids is not more than 17 area percent.