US2013121972A1PendingUtilityA1

Native wharton's jelly stem cells and their purification

59
Individually held — no corporate assignee on recordPriority: Jun 1, 2010Filed: Jun 1, 2011Published: May 16, 2013
Est. expiryJun 1, 2030(~3.8 yrs left)· nominal 20-yr term from priority
Inventors:Rouzbeh R. Taghizadeh
C12N 5/06A61L 27/3834C12N 2500/84C12N 5/0662C12N 5/0605C12N 5/0668C12N 5/0602C12N 5/0037C12N 5/00C12N 2502/025A61K 35/51
59
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Claims

Abstract

Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Claims

exact text as granted — not AI-modified
We claim: 
     
         1 . A method of purifying noncultured Wharton's Jelly stem cells, the method comprising separating noncultured Wharton's Jelly stem cells from a digested tissue comprising Wharton's Jelly. 
     
     
         2 . The method of  claim 1 , further comprising digesting a tissue comprising Wharton's Jelly. 
     
     
         3 - 5 . (canceled) 
     
     
         6 . A method according to  claim 2 , wherein the tissue is digested by mechanically increasing the surface area of the tissue. 
     
     
         7 . The method of  claim 6 , comprising cutting the tissue. 
     
     
         8 . The method of  claim 6 , comprising mincing the tissue. 
     
     
         9 . (canceled) 
     
     
         10 . A method according to  claim 2 , further comprising separating digested and undigested tissue. 
     
     
         11 . The method of  claim 10 , comprising sedimenting the undigested tissue. 
     
     
         12 . The method of  claim 11 , wherein centrifugation is used for sedimenting the undigested tissue. 
     
     
         13 . The method of  claim 10 , comprising filtering the digested tissue. 
     
     
         14 . The method of  claim 13 , wherein the noncultured Wharton's Jelly stem cells are separated from the filtrate. 
     
     
         15 . A method according to  claim 10 , further comprising washing or diluting the digested tissue before separating it from the undigested tissue. 
     
     
         16 . A method of purifying cultured and noncultured Wharton's Jelly stem cells, the method comprising:
 purifying noncultured Wharton's Jelly stem cells according to  claim 10 ; and   culturing mesenchymal stem cells from the undigested tissue.   
     
     
         17 . The method of  claim 16 , wherein the mesenchymal stem cells are cultured in digested Wharton's Jelly. 
     
     
         18 . Purified, noncultured Wharton's Jelly stem cells substantially free of semi-solid Wharton's Jelly. 
     
     
         19 . Purified, noncultured Wharton's Jelly stem cells at a temperature below −20° C. 
     
     
         20 . Wharton's Jelly stem cells according to  claim 18 , wherein the cells have reduced CD73 and reduced CD105 levels compared to cultured mesenchymal stem cells. 
     
     
         21 . Wharton's Jelly stem cells according to  claim 18 , wherein the Wharton's Jelly stem cells are maintained in a sterile solution. 
     
     
         22 . (canceled) 
     
     
         23 . A method of treating a subject, the method comprising administering to the subject Wharton's Jelly stem cells according to  claim 18 . 
     
     
         24 . (canceled) 
     
     
         25 . A homogeneous solution comprising Wharton's Jelly. 
     
     
         26 - 28 . (canceled) 
     
     
         29 . A method of maintaining a cell comprising mixing the cell in the solution of  claim 25 . 
     
     
         30 - 32 . (canceled)

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