US2013116404A1PendingUtilityA1

Targeted non-invasive imaging probes of egfr expressing cells

Assignee: UNIV CASE WESTERN RESERVEPriority: Nov 8, 2011Filed: Nov 8, 2012Published: May 9, 2013
Est. expiryNov 8, 2031(~5.3 yrs left)· nominal 20-yr term from priority
C07K 7/56C07K 7/08
48
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

A probe for imaging EGFR expressing cells includes an EGFR targeting moiety, a reporter moiety, and a hydrophilic linker that links the EGFR targeting moiety to the reporter moiety. The hydrophilic linker enhances solubility of the probe in an aqueous media as well as binding affinity of the probe to EGFR expressing cells.

Claims

exact text as granted — not AI-modified
Having described the invention, we claim: 
     
         1 . A probe for imaging EGFR expressing cells, comprising:
 an EGFR targeting moiety,   a reporter moiety, and   a hydrophilic linker that links the EGFR targeting moiety to the reporter moiety, the hydrophilic linker enhancing solubility of the probe in an aqueous media as well as binding affinity of the probe to EGFR expressing cells.   
     
     
         2 . The probe of  claim 1 , EGFR targeting moiety comprising a peptide having SEQ ID NO: 1 or SEQ ID NO: 2. 
     
     
         3 . The probe of  claim 1 , the reporter moiety comprising a fluorochrome. 
     
     
         4 . The probe of  claim 1 , the linker comprising a polyethylene glycol linker. 
     
     
         5 . The probe of  claim 1 , upon systemic administration to subject being capable of crossing the blood brain barrier. 
     
     
         6 . The probe of  claim 1 , the EGFR expressing cells comprising EGFR expressing cancer cells. 
     
     
         7 . The probe of  claim 1 , comprising the following formula: 
       
         
           
           
               
               
           
         
         where R is a near infrared fluorescence moiety and n is an integer from 1-3. 
       
     
     
         8 . A probe for imaging EGFR expressing cells, comprising:
 a hydrophobic EGFR targeting moiety,   a reporter moiety, and   a hydrophilic linker that links the hydrophobic EGFR targeting moiety to the reporter moiety, the hydrophilic linker enhancing solubility of the probe in an aqueous media as well as binding affinity of the probe to EGFR expressing cells.   
     
     
         9 . The probe of  claim 8 , EGFR targeting moiety comprising a peptide having SEQ ID NO: 1 or SEQ ID NO: 2. 
     
     
         10 . The probe of  claim 9 , the reporter moiety comprising a fluorochrome. 
     
     
         11 . The probe of  claim 9 , the linker comprising a polyethylene glycol linker. 
     
     
         12 . The probe of  claim 9 , upon systemic administration to subject being capable of crossing the blood brain barrier. 
     
     
         13 . The probe of  claim 9 , the EGFR expressing cells comprising EGFR expressing cancer cells. 
     
     
         14 . The probe of  claim 8 , comprising the following formula: 
       
         
           
           
               
               
           
         
         where R is a near infrared fluorescence moiety and n is an integer from 1-3. 
       
     
     
         15 . A probe for imaging EGFR expressing cells, comprising:
 a hydrophobic EGFR targeting moiety that includes a peptide having SEQ ID NO: 1 or SEQ ID NO: 2,   a fluorochrome reporter moiety, and   a hydrophilic linker that links the hydrophobic EGFR targeting moiety to the reporter moiety, the hydrophilic linker enhancing solubility of the probe in an aqueous media as well as binding affinity of the probe to EGFR expressing cells.   
     
     
         16 . The probe of  claim 15 , the linker comprising a polyethylene glycol linker. 
     
     
         17 . The probe of  claim 15 , upon systemic administration to subject being capable of crossing the blood brain barrier. 
     
     
         18 . The probe of  claim 15 , the EGFR expressing cells comprising EGFR expressing cancer cells. 
     
     
         19 . The probe of  claim 15 , comprising the following formula: 
       
         
           
           
               
               
           
         
         where R is a near infrared fluorescence moiety and n is an integer from 1-3. 
       
     
     
         20 . The probe of  claim 15 , selected from the group consisting of: 
       
         
           
           
               
               
           
         
         where n is an integer from 1 to 3.

Join the waitlist — get patent alerts

Track US2013116404A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.