Bone semi-permeable device

Abstract

Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.

1 .- 147 . (canceled) 
     
     
         148 . A method of making a device, comprising:
 combining an implantable bone structure configured to form an internal cavity and a semi-permeable component composed of one or more fused red blood cell ghosts, such that an internal cavity is maintained.   
     
     
         149 . The method of  claim 148 , wherein one or more biologically active molecules are encapsulated in the one or more fused red blood cell ghosts. 
     
     
         150 . A method of making a device, comprising:
 exposing an implantable bone structure configured to form an internal cavity to platelets, wherein the implantable bone structure is at least partially coated with a platelet aggregating compound.   
     
     
         151 . The method of  claim 150 , wherein the implantable bone structure is exposed to platelets in vitro. 
     
     
         152 . The method of  claim 150 , wherein the implantable bone structure is exposed to platelets in vivo. 
     
     
         153 . The method of  claim 150 , wherein the internal cavity of the implantable bone structure is at least partially coated with the platelet aggregating compound. 
     
     
         154 . The method of  claim 150 , wherein an outer surface of the implantable bone structure is at least partially coated with the platelet aggregating compound. 
     
     
         155 . A method of making a device, comprising:
 receiving an implantable porous bone structure that includes an internal cavity therein and a plurality of pores that are in communication with the internal cavity, wherein the implantable porous bone structure includes at least one of organic, anorganic, demineralized, or freeze-dried bone;   receiving a semi-permeable membrane, wherein the semi-permeable membrane is substantially continuous and capable of covering multiple ones of the plurality of pores of the implantable porous bone structure, and further wherein the semi-permeable membrane includes one or more fused red blood cell ghosts; and   combining the implantable porous bone structure and the semi-permeable membrane such that the semi-permeable membrane covers the multiple ones of the plurality of pores of the implantable porous bone structure, the semi-permeable membrane positioned and configured to allow one or more biologically active substances disposed in the internal cavity to pass through the semi-permeable membrane and out of the implantable porous bone structure while the semi-permeable membrane covers the multiple ones of the plurality of pores of the implantable porous bone structure.   
     
     
         156 . The method of  claim 155 , further comprising:
 wherein combining an implantable porous bone structure and a semi-permeable membrane includes lining the internal cavity with the semi-permeable membrane; and   disposing the one or more biological active substances in the internal cavity of the implantable porous bone structure.   
     
     
         157 . The method of  claim 155 , wherein combining an implantable porous bone structure and a semi-permeable membrane includes at least partially surrounding the implantable porous bone structure with the semi-permeable membrane. 
     
     
         158 . The method of  claim 155 , wherein the implantable porous bone structure includes living bone cells in artificial bone scaffolding. 
     
     
         159 . The method of  claim 155 , wherein the semi-permeable membrane is separately formed from the implantable porous bone structure prior to combining the implantable porous bone structure and the semi-permeable membrane. 
     
     
         160 . A method of making a device, comprising:
 receiving an implantable porous bone structure that includes an internal cavity therein and a plurality of pores that are in communication with the internal cavity, wherein the implantable porous bone structure includes at least one of organic, anorganic, demineralized, or freeze-dried bone, and further wherein the implantable porous bnoe structure is at least partially coated with a platelet aggregating compound;   exposing the implantable porous bone structure to platelets to form a semi-permeable component, wherein the semi-permeable component is substantially continuous and capable of covering multiple ones of the plurality of pores of the implantable porous bone structure, and further wherein the semi-permeable membrane includes one or more aggregated platelets; and   wherein the semi-permeable component covers the multiple ones of the plurality of pores of the implantable porous bone structure, the semi-permeable membrane positioned and configured to allow one or more biologically active substances disposed in the internal cavity to pass through the semi-permeable component and out of the implantable porous bone structure while the semi-permeable component covers the multiple ones of the plurality of pores of the implantable porous bone structure.   
     
     
         161 . The method of  claim 160 , wherein the implantable porous bone structure comprises living bone cells in artificial bone scaffolding. 
     
     
         162 . The method of  claim 160 , wherein the implantable porous bone structure is exposed to platelets in vitro. 
     
     
         163 . The method of  claim 160 , wherein the implantable porous bone structure is exposed to platelets in vivo. 
     
     
         164 . The method of  claim 160 , wherein the internal cavity of the implantable porous bone structure is at least partially coated with the platelet aggregating compound. 
     
     
         165 . The method of  claim 160 , wherein an outer surface of the implantable porous bone structure is at least partially coated with the platelet aggregating compound.