Isoforms of the human sst5 receptor originated by alternative splicing and oligonucleotide pairs to detect them by pcr

Abstract

The present invention is referred to two human nucleic acids that comprise sequences encoding two new isoforms of the human somatostatin receptor type 5 originated by alternative splicing, named sst5B and sst5C, with possible involvement in tumor processes. In addition, the invention is referred to oligonucleotide pairs allowing their differential detection in several tissues using the PCR technique.

1 . Purified somatostatin receptor type 5 oligonucleotides, fragments, homologs, or fully complementary nucleic acids thereof, wherein
 a) said purified oligonucleotide, fragment, homolog or fully complementary nucleic acid comprises a sequence having at least 90% identity with SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14, and   b) said purified oligonucleotide, fragment, homolog or fully complementary nucleic acid is capable as acting as a primer for the PCR amplification of a human somatostatin receptor type 5 nucleic acid.   
     
     
         2 . The purified oligonucleotides, fragments, homologs, or fully complementary nucleic acids according to  claim 1 , wherein said human somatostatin receptor type 5 nucleic acid comprises a sequence having at least 90% identity with SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7. 
     
     
         3 . The purified oligonucleotides, fragments, homologs, or fully complementary nucleic acids according to  claim 2 , wherein said human somatostatin receptor type 5 nucleic acid is SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7. 
     
     
         4 . The purified oligonucleotides, fragments, homologs, or fully complementary nucleic acids according to  claim 3 , wherein said purified oligonucleotide, fragment, homolog or complementary nucleic acid is SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14. 
     
     
         5 . A method of selectively amplifying a human somatostatin receptor type 5 nucleic acid, wherein purified oligonucleotides, fragments, homologs, or fully complementary nucleic acids thereof comprising a sequence having at least 90% identity with SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14, are used for the PCR amplification of a human somatostatin receptor type 5 nucleic acid. 
     
     
         6 . A method according to  claim 5 , wherein said amplified human somatostatin receptor type 5 nucleic acid comprises a sequence having at least 90% identity with SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7. 
     
     
         7 . A method according to  claim 6 , wherein said amplified human somatostatin receptor type 5 nucleic acid is SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7. 
     
     
         8 . A method according to  claim 5 , wherein said purified oligonucleotides, fragments, homologs, or fully complementary nucleic acids thereof are selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14, and wherein said amplified human somatostatin receptor type 5 nucleic acid is SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7. 
     
     
         9 . A method of determining the tissue distribution of human somatostatin receptor type 5 nucleic acids utilizing purified oligonucleotides comprising a sequence having at least 90% identity with SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14, or purified fragments, homologs, or fully complementary nucleic acids thereof. 
     
     
         10 . A method of gene silencing the expression of either or both sst5B or sst5C somatostatin receptor type 5 genes, said method comprising administering a somatostatin receptor type 5 nucleic acid, fragment, homolog or fully complementary nucleic acid comprising a sequence having at least 90% homology to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7. 
     
     
         11 . The use of a somatostatin receptor type 5 polypeptide, fragment or homolog thereof for the production of antibodies, wherein
 a) said somatostatin receptor type 5 polypeptide, fragment or homolog shares at least 90% identity with SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8, and wherein   b) said antibodies discriminate sst5B and sst5C polypeptide isoforms.