Phenolic binding peptides

Abstract

The present application relates to peptides which bind to tannin, polyphenolic or anthocyanin compounds, and particularly to tea and wine stains on a fabric or other surface. The invention also concerns binding peptide conjugates which includes a binding peptide coupled to an agent and the use of the binding peptide conjugate for delivering an agent to a desired target.

1 . A binding peptide comprising an amino acid sequence shown in any one of SEQ ID NOs. 1-316 and a binding peptide having at least 70% sequence identity thereto. 
     
     
         2 . (canceled) 
     
     
         3 . The binding peptide of  claim 1 , wherein the peptide is selected from the group consisting of KTPSPHG (SEQ ID NO. 1); PNTTRHS (SEQ ID NO. 2); LWTSPQL (SEQ ID NO. 8); TNNTSPT (SEQ ID NO. 24); SPTSTNS (SEQ ID NO. 43); TTTTPFA (SEQ ID NO. 77); SWNTSPL (SEQ ID NO. 80); QAVKASHATMYL (SEQ ID NO. 97); SYDLIPPRSGLA (SEQ ID NO. 104); DPNTTSH (SEQ ID NO.118); KASHLVP (SEQ ID NO: 132); LPTSTLT (SEQ ID NO. 139); QNQKSTT (SEQ ID NO. 158); SIIPPRQ (SEQ ID NO. 168); WSNKPLSPNDLR (SEQ ID NO. 193) and peptides having at least 75% amino acid sequence identity thereto. 
     
     
         4 . The binding peptide of  claim 1 , having a repeatable motif selected from the group consisting of
 LPL (SEQ ID NOs. 120, 123, 115 and 250);   FAT (SEQ ID NOs. 125, 227 and 235);   STT (SEQ ID NOs. 90, 158, 230 and 310);   HSP (SEQ ID NOs. 18, 252 and 307);   TNK (SEQ ID NOs. 40, 259 and 287);   SPL (SEQ ID NOs. 53, 80, 152, 229, 232 and 292);   THS (SEQ ID NOs. 62, 209 and 290);   TSP (SEQ ID NOs. 8, 24, 80, 223 and 291);   SPT (SEQ ID NOs. 24, 43 and 266);   AQT (SEQ ID NOs. 59, 134 and 205);   NSS (SEQ ID NOs. 31, 86, 213, 227 and 278);   PAL (SEQ ID NOs. 109, 224 and 256);   SGL (SEQ ID NOs. 104, 284 and 298); and   TQT (SEQ ID NOs. 105, 281 and 287) and a binding peptide having the repeatable motif and at least 75% amino acid sequence identity to a binding peptide having the repeatable motif and listed herein.   
     
     
         5 . The binding peptide of  claim 1 , wherein said peptide binds to a compound selected from the group consisting of tannin, anthocyanin and phenolic compounds. 
     
     
         6 . The binding peptide of  claim 1 , wherein said peptide binds to a tea or wine stain. 
     
     
         7 . The binding peptide of  claim 6 , wherein the peptide binds to a tea or wine stain on a fabric. 
     
     
         8 . The binding peptide of  claim 6 , wherein the peptide binds to a tea or wine stain on a surface. 
     
     
         9 . The binding peptide of  claim 8 , wherein the surface is selected from the group consisting of ceramic, glass, wood, paper, skin, hair and plastic. 
     
     
         10 . The binding peptide of  claim 1 , further comprising a cysteine amino acid residue at the N and C terminus of said peptide. 
     
     
         11 . A binding peptide conjugate comprising a binding peptide of  claim 1 , linked to an agent. 
     
     
         12 . (canceled) 
     
     
         13 . The conjugate according to  claim 11 , wherein said agent is a protein. 
     
     
         14 . The conjugate of  claim 13 , wherein the protein is an enzyme. 
     
     
         15 . The conjugate of  claim 14 , wherein said enzyme is an enzyme that catalyzes an oxidation-reduction reaction and is selected from the group consisting of laccases, phenol oxidases, catalases, bilirubrin oxidases, glucose oxidases, and peroxidases. 
     
     
         16 . The conjugate of  claim 11 , wherein said binding peptide is covalently linked to said agent. 
     
     
         17 . The conjugate of  claim 11 , wherein said binding peptide and said agent are separated by a linker. 
     
     
         18 - 25 . (canceled) 
     
     
         26 . A method for delivering an agent to a target comprising a) conjugating an agent with a binding peptide of  claim 1  to form a binding peptide conjugate and b) exposing a target to the binding peptide conjugated, wherein the binding peptide conjugate binds to said target. 
     
     
         27 . The method according to  claim 26 , wherein the target is a tea or wine stain. 
     
     
         28 . The method according to  claim 26 , wherein the agent is an enzyme. 
     
     
         29 - 35 . (canceled)