US2013075663A1PendingUtilityA1

Nucleic acid ladders

44
Individually held — no corporate assignee on recordPriority: Jul 15, 1997Filed: Aug 21, 2012Published: Mar 28, 2013
Est. expiryJul 15, 2017(expired)· nominal 20-yr term from priority
Inventors:Heather J. Jordan
C12N 15/11C12Q 1/68C12Q 1/6876Y10T436/143333
44
PatentIndex Score
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Claims

Abstract

The present invention provides nucleic acid compositions or ladders which may be used as standards for estimating the size (in base pairs) and or mass of nucleic acid molecules of unknown size and/or mass. The invention also relates to methods for producing such compositions or ladders, ladders or compositions produced by such methods, and to methods for estimating the size and/or mass of nucleic acid molecules by comparison to these nucleic acid sizing ladders.

Claims

exact text as granted — not AI-modified
1 . A nucleic acid ladder comprising a mixture of nucleic acid fragments of different lengths, wherein the relative mass of each nucleic acid fragment is substantially equivalent relative to the other fragments, and wherein said different length fragments produce bands after gel electrophoresis, said bands having substantially equal intensity when visualized. 
     
     
         2 . (canceled) 
     
     
         3 . A nucleic acid ladder composition comprising a mixture of nucleic acid fragments of different sizes, wherein the differently sized fragments are capable of producing bands after gel electrophoresis and staining, said bands having substantially equal intensity. 
     
     
         4 . The composition or ladder according to  claim 3 , wherein said nucleic acid in DNA. 
     
     
         5 . The composition or ladder according to  claim 1 , wherein each of said fragments differ in length by X base pairs, wherein x is an integer equal to or greater than 10. 
     
     
         6 . The composition or ladder a cording to  claim 5 , wherein x is an integer equal to or greater than 100. 
     
     
         7 . The composition or ladder according to  claim 1 , wherein said mixture comprises fragments having two or more increments of about 1 kb in length. 
     
     
         8 . The composition or ladder according to  claim 7 , wherein said fragments range in size from about 25 kb to about 1 kb. 
     
     
         9 . The composition or ladder according to  claim 1 , wherein said fragments are multiples of about 1 kb. 
     
     
         10 . The composition or ladder according to  claim 1 , wherein said mixture comprises fragments having one or more increments selected from the group consisting of, about 50 bp in length, and about 10 bp in length, about 100 bp in length, about 500 bp in length, and about 1000 bp in length. 
     
     
         11 . The composition or ladder according to  claim 7 , further comprising fragments having one or more increments selected from the group consisting of about 100 bp in length, about 500 bp in length, about 50 bp in length, and about 10 bp in length. 
     
     
         12 . The composition or ladder according to  claim 7 , further comprising fragments having one or more multiples selected from the group consisting of about 100 bp in length, about 500 bp in length, about 50 bp in length, and about 10 bp in length. 
     
     
         13 . The composition or ladder according to  claim 11 , which comprises one or more fragments at a relative mass sufficient to produce one or more highlight bands after gel electrophoresis and staining. 
     
     
         14 . The composition or ladder according to  claim 12  which comprises one or more fragments at a relative mass sufficient to produce one or more highlight bands after gel electrophoresis and staining. 
     
     
         15 . The composition or ladder according to  claim 1 , wherein one or more of said nucleic acid fragments are single stranded. 
     
     
         16 . The composition or ladder according to  claim 1 , wherein one or more of aid nucleic acid fragments are double stranded. 
     
     
         17 . The composition or ladder according to  claim 16 , wherein said double stranded fragments are blunt ended. 
     
     
         18 . The composition or ladder according  claim 16 , wherein said double stranded fragments comprise a single stranded overhang at each terminus. 
     
     
         19 . (canceled) 
     
     
         20 . (canceled) 
     
     
         21 . The composition or ladder according to  claim 1 , wherein said relative mass of each fragment is no more than about 3 times the relative mass of a other fragment. 
     
     
         22 . The composition or ladder according to  claim 1 , wherein said relative mass of each fragment is no more than about 2 times the relative mass of another fragment. 
     
     
         23 . The composition or ladder according to  claim 1 , wherein said relative mass of each fragment is no more than about 1.5 times the relative mass of another fragment. 
     
     
         24 . The composition or ladder according to  claim 1 , wherein said mass of each fragment is about the same. 
     
     
         25 . The composition or ladder according to  claim 1 , wherein said fragments are detectable labeled. 
     
     
         26 . The composition or ladder according to  claim 25 , wherein said detectable label is selected from the group consisting of a radiolabel, a fluorescent label, and a chemiluminescent label. 
     
     
         27 . The composition or ladder according to  claim 4 , wherein said mixture of DNA fragments comprises about 9 copies of a 100 bp fragment, about 4 copies of a 200 bp fragment, about 3 copies of a 400 bp fragment, about 2 copies of a 500 bp fragment, about 1 copy of a 650 bp fragment, about 1 copy of an 850 bp fragment, about 12 copies of 1000 bp fragment, and about 1 copy of 1650 bp fragment. 
     
     
         28 - 42 . (canceled)

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