Composition and method for attraction of emerald ash borer agrilus planipennis fairmaire (coleoptera: buprestidae)

Abstract

The invention disclosed relates to a novel composition and use thereof, for the attraction and detection of emerald ash borer, Agrilus planipennis Fairmaire. The composition comprises (3Z)-dodecen-12-olide and ash foliar or cortical volatiles (green leaf volatiles) associated with a prism trap of a color in the green range of the visible light spectrum. A significant increase in the capture of male A. planipennis is achieved when traps were deployed in the upper tree canopy. This invention is the first demonstration of increased attraction with a combination of a pheromone and a green leaf volatile in a Buprestid species.

1 . A composition for the attraction of  A. planipennis , comprising
 (a) (3Z)-dodecen-12-olide and   (b) ash foliar volatiles, associated with a trap of a color in the green range of the visible light spectrum.   
     
     
         2 . A composition according to  claim 1 , wherein the ash foliar volatiles comprise (3Z)-hexenol. 
     
     
         3 . A composition according to  claim 2 , wherein the trap is a prism sticky trap of a green color defined by a wavelength of 540-560 nm and a reflectance of 24-64%. 
     
     
         4 . A composition according to  claim 2 , wherein the trap includes an insecticide. 
     
     
         5 . A composition according to  claim 2 , wherein the amount of (3Z)-dodecen-12-olide is a source dosage which emits 2.4-160 μg of (3Z)-dodecen-12-olide per day, and wherein the amount of (3Z)-hexenol is a source dosage which emits 50-100 mg of (3Z)-hexenol per day, at about 25° C. 
     
     
         6 . A composition according to  claim 5 , wherein the amount of (3Z)-dodecen-12-olide emitted is 2.4-22 μg/day and the amount of (3Z)-hexenol emitted is 40-60 mg/day, at about 25° C., and wherein the green color is defined by a wavelength of 540 nm and a reflectance of 49%. 
     
     
         7 . A composition according to  claim 6 , wherein the amount of (3Z)-dodecen-12-olide emitted is 2.4 μg/day at 25° C. 
     
     
         8 . A method for the attraction of  A. planipennis , comprising applying to an insect habitat an insect attracting amount of (3Z)-dodecen-12-olide and ash foliar volatiles, associated with a trap of a color in the green range of the visible light spectrum. 
     
     
         9 . A method according to  claim 8 , wherein the insect habitat is a tree canopy. 
     
     
         10 . A method according to  claim 9 , wherein the tree canopy is the upper tree canopy. 
     
     
         11 . A method according to  claim 8 , wherein the ash foliar volatiles comprise 3(Z)-hexenol. 
     
     
         12 . A method according to  claim 11 , wherein the trap is a prism sticky trap of a green color defined by a wavelength of 540-560 nm and a reflectance of 24-64%. 
     
     
         13 . A method according to  claim 11 , wherein the trap is of a green color defined by a wavelength of about 540 nm and a reflectance of 49%, and includes an insecticide. 
     
     
         14 . A method according to  claim 11 , wherein the amount of (3Z)-dodecen-12-olide is a source dosage which emits 2.4-160 μg of (3Z)-dodecen-12-olide per day, and wherein the amount of (3Z)-hexenol is a source dosage which emits 50-100 mg of (3Z)-hexenol per day, at about 25° C. 
     
     
         15 . A method according to  claim 14 , wherein the amount of (3Z)-dodecen-12-olide emitted is 2.4-22 μg per day and the amount of (3Z)-hexenol emitted is 40-60 mg per day. 
     
     
         16 . A method according to  claim 15 , wherein the amount of (3Z)-dodecen-12-olide emitted is 2.4 μg/day at 25° C. 
     
     
         17 . A kit for the attraction of  A. planipennis  comprising:
 (a) a spectrum containing an insect attracting amount of (3Z)-dodecen-12-olide dissolved in a volatile solvent, which evaporates before use leaving the (3Z)-dodecen-12-olide to emit therefrom,   (b) a bubble cap containing an effective amount of neat ash green volatiles, maintained separately until use, and   (c) a trap of a color within the green range of the visible light spectrum.   
     
     
         18 . A kit according to  claim 17 , wherein the amount of (3Z)-dodecen-12-olide is a source dosage which emits 2.4-160 μg of (3Z)-dodecen-12-olide per day. 
     
     
         19 . A kit according to  claim 18 , wherein the ash foliar volatiles comprise (3Z)-hexenol of a source dosage which emits 50-100 mg of (3Z)-hexenol per day. 
     
     
         20 . A kit according to  claim 19 , wherein the amount of (3Z)-dodecen-12-olide emitted is 2.4-22 μg per day and the amount of (3Z)-hexenol emitted is 40-60 mg per day.