Methods for Detecting Defects in Inorganic-Coated Polymer Surfaces

Abstract

Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.

1 . A method of identifying a defect in a surface, comprising:
 a) providing a substrate having a hydrophobic surface at least partially coated by a hydrophilic layer, wherein the hydrophilic layer has the defect therein;   b) contacting the substrate with a lipophilic, fluorescent substance, for a sufficient amount of time for the substance to contact the defect;   c) exciting the fluorescent substance with energy at an appropriate wavelength to generate a detectable fluorescence response; and,   d) detecting the fluorescence response of the substance.   
     
     
         2 . The method of  claim 1 , further comprising washing the substrate after contacting the substrate with the lipophilic, fluorescent substance. 
     
     
         3 . The method of  claim 1  wherein the substrate comprises a polymer. 
     
     
         4 . The method of  claim 1  wherein the hydrophilic layer is or comprises an inorganic material. 
     
     
         5 . The method of  claim 1  wherein the lipophilic, fluorescent substance is a fluorescent compound that comprises a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene moiety. 
     
     
         6 . The method of  claim 1  wherein the lipophilic, fluorescent substance further comprises a lipophilic moiety. 
     
     
         7 . The method of  claim 6  wherein the lipophilic, fluorescent substance further comprises two or more lipophilic moieties. 
     
     
         8 . The method of  claim 6  wherein the lipophilic moiety is a hydrocarbon having 1-20 carbon atoms. 
     
     
         9 . The method of  claim 6  wherein the lipophilic moiety is an alkyl group having 1-20 carbon atoms. 
     
     
         10 . The method of  claim 6  wherein the lipophilic moiety is phenyl or styryl group. 
     
     
         11 . The method of  claim 1  wherein the hydrophilic layer is less than 10 Å in thickness. 
     
     
         12 . The method of  claim 1  wherein the lipophilic, fluorescent substance is associated with a microparticle. 
     
     
         13 . The method of  claim 1  wherein the lipophilic, fluorescent substance is a semiconductor nanocrystal. 
     
     
         14 . A lipophilic, fluorescent substance that comprises a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene moiety and a lipophilic moiety. 
     
     
         15 . The lipophilic, fluorescent substance of  claim 14  wherein the lipophilic moiety is a hydrocarbon having 1-20 carbon atoms. 
     
     
         16 . The lipophilic, fluorescent substance of  claim 14  wherein the lipophilic moiety is an alkyl group having 1-20 carbon atoms. 
     
     
         17 . The lipophilic, fluorescent substance of  claim 14  wherein the lipophilic moiety is a phenyl or styryl group. 
     
     
         18 . The lipophilic, fluorescent substance of  claim 14  wherein the substance is associated with a microparticle. 
     
     
         19 . A substrate comprising an identifiable defect:
 a) a hydrophobic surface, a substrate having a hydrophobic surface at least partially coated by a hydrophilic layer, wherein the hydrophilic layer has the defect therein; and   b. a lipophilic, fluorescent substance that is in contact with the defect.   
     
     
         20 . The substrate of  claim 19  wherein the lipophilic, fluorescent substance comprises a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene moiety and a lipophilic moiety.