Biosensor Having Improved Hematocrit and Oxygen Biases

Abstract

The present invention provides reagent compositions, and analyte measuring devices and methods that utilize the reagent compositions.

1 - 65 . (canceled) 
     
     
         66 . A reagent composition, comprising:
 a mediator comprising a phenanthroline quinone or a derivative thereof;   a transitional metal ion or heavier alkaline earth metal ion; and   a counter ion.   
     
     
         67 . The reagent composition of  claim 66 , wherein the phenanthroline quinone is a 1,10-phenanthroline quinone. 
     
     
         68 . The reagent composition of  claim 66 , wherein the transition metal ion is selected from the group consisting of nickel, manganese, iron, cobalt, osmium and ruthenium. 
     
     
         69 . The reagent composition of  claim 68 , wherein the transition metal ion is nickel. 
     
     
         70 . The reagent composition of  claim 66 , wherein the heavier earth metal ion is selected from the group consisting of calcium, strontium and barium. 
     
     
         71 . The reagent composition of  claim 66 , wherein the counter anion is a halide, a nitrate, a nitrite, a sulfate, a carbonate, a phosphate, a thiocyanate, an acetate, a formate, a citrate, a succinate, an oxalate, a tartrate, a benzoate, or an alkyl or aromatic sulfonate. 
     
     
         72 . The reagent composition of  claim 71 , wherein the halide is chloride, bromide, fluoride, or iodide. 
     
     
         73 . The reagent composition of  claim 66 , wherein the counter anion is a tungstate, a molybdate, a ferricyanide, a nitroprusside, a tetraphenylborate, an anionic dye or an anionic surfactant. 
     
     
         74 . The reagent composition of  claim 66 , wherein the reagent composition further comprises an enzyme. 
     
     
         75 . The reagent composition of  claim 74 , wherein the enzyme is NAD(P) + -dependent dehydrogenase. 
     
     
         76 . The reagent composition of  claim 74 , wherein the enzyme is selected from the group consisting of alcohol dehydrogenase, lactate dehydrogenase, glucose oxidase, glucose dehydrogenase, cholesterol oxidase and 3-hydroxybutyrate dehydrogenase. 
     
     
         77 . The reagent composition of  claim 74 , wherein the enzyme is 3-hydroxybutyrate dehydrogenase. 
     
     
         78 . A biosensor comprising:
 an electrode support;   a working electrode disposed on the electrode support;   a reference electrode disposed on the electrode support; and   a reagent composition deposited over the working electrode and the reference electrode, wherein the reagent composition comprises:
 an enzyme; 
 a mediator comprising a phenanthroline quinone or a derivative thereof; 
 a transitional metal ion or heavier alkaline earth metal ion; and 
 a counter ion. 
   
     
     
         79 . The biosensor of  claim 78 , wherein the biosensor further comprises a counter electrode. 
     
     
         80 . The biosensor of  claim 78 , wherein the biosensor further comprises a cover layer defining an enclosed space over the electrodes, the cover layer having an aperture configured for receiving a sample into the enclosed space. 
     
     
         81 . The biosensor of  claim 78 , wherein the phenanthroline quinone is a 1,10-phenanthroline quinone. 
     
     
         82 . The biosensor of  claim 78 , wherein the enzyme is selected from the group consisting of alcohol dehydrogenase, lactate dehydrogenase, glucose oxidase, glucose dehydrogenase, cholesterol oxidase and 3-hydroxybutyrate dehydrogenase. 
     
     
         83 . The biosensor of  claim 82 , wherein the enzyme is 3-hydroxybutyrate dehydrogenase. 
     
     
         84 . The biosensor of  claim 78 , wherein the transition metal ion is selected from the group consisting of nickel, manganese, iron, cobalt, osmium and ruthenium. 
     
     
         85 . The biosensor of  claim 84 , wherein the transition metal ion is nickel. 
     
     
         86 . The biosensor of  claim 78 , wherein the heavier earth metal ion is selected from the group consisting of calcium, strontium and barium. 
     
     
         87 . The biosensor of  claim 78 , wherein the counter anion is a halide, a nitrate, a nitrite, a sulfate, a carbonate, a phosphate, a thiocyanate, an acetate, a formate, a citrate, a succinate, an oxalate, a tartrate, a benzoate, or an alkyl or aromatic sulfonate. 
     
     
         88 . The biosensor of  claim 87 , wherein the halide is chloride, bromide, fluoride, or iodide. 
     
     
         89 . The biosensor of  claim 78 , wherein the counter anion is a tungstate, a molybdate, a ferricyanide, a nitroprusside, a tetraphenylborate, an anionic dye or an anionic surfactant.