Adipose tissue matrices

Abstract

The present disclosure provides tissue products produced from adipose tissues, as well as methods for producing such tissue products. The tissue products can include acellular extracellular matrices. In addition, the present disclosure provides systems and methods for using such products.

1 . A method for producing a tissue product, comprising:
 selecting an adipose tissue;   mechanically processing the adipose tissue to reduce the tissue size;   treating the mechanically processed tissue to remove substantially all cellular material from the tissue;   suspending the tissue in a solution; and   cross-linking the tissue to produce a stable three-dimensional structure.   
     
     
         2 . The method of  claim 1 , further including drying the tissue. 
     
     
         3 . The method of  claim 2 , wherein the tissue is dried after being mechanically processed and suspended but before being cross-linking. 
     
     
         4 . The method of  claim 2 , wherein drying includes freeze-drying. 
     
     
         5 . The method of  claim 1 , wherein cross-linking includes contacting the tissue with a cross-linking agent. 
     
     
         6 . The method of  claim 5 , wherein the cross-linking agent includes at least one of glutaraldehyde, genepin, carbodiimides, and diisocyantes. 
     
     
         7 . The method of  claim 1 , wherein cross-linking includes heating the tissue. 
     
     
         8 . The method of  claim 7 , wherein the tissue is heated in a vacuum. 
     
     
         9 . The method of  claim 7 , wherein the tissue is heated to 70° C. to 120° C. 
     
     
         10 . The method of  claim 1 , further comprising placing the tissue in a mold to produce a desired shape prior to cross linking. 
     
     
         11 . The method of  claim 1 , wherein the tissue is cross-linked such that the material maintains the stable three-dimensional structure when contacted with an aqueous environment. 
     
     
         12 . The method of  claim 11 , wherein the material maintains the stable three-dimensional structure when implanted in a body. 
     
     
         13 . The method of  claim 1 , wherein mechanically processing the tissue includes at least one of cutting, grinding, or blending to produce small particles. 
     
     
         14 . The method of  claim 1 , further comprising contacting the tissue with a flat surface and applying force to compress the tissue to a thickness of about 2.0 mm or less and optionally cross-linking the tissue. 
     
     
         15 . The method of  claim 1 , further comprising processing the tissue to form a drain or drain manifold in the form of a tube, column, or sheet, further processing the tissue to add grooves, channels, or holes, and cross-linking the tissue. 
     
     
         16 . The method of  claim 1 , further comprising processing the tissue to form thin strips have a thickness of up to about 2.0 mm, rolling the thin strips to form a drain in the shape of a hollow tube, and cross-linking the tissue. 
     
     
         17 . A tissue product made by a process comprising:
 selecting an adipose tissue;   mechanically processing the adipose tissue to reduce the tissue size;   treating the processed tissue to remove substantially all cellular material from the tissue;   suspending the tissue in a solution; and   cross-linking the tissue to produce a stable three-dimensional structure.   
     
     
         18 . The product of  claim 17 , wherein the process further includes drying the tissue. 
     
     
         19 . The product of  claim 18 , wherein the tissue is dried after being mechanically processed and suspended but before being cross-linking. 
     
     
         20 . The product of  claim 18 , wherein drying includes freeze-drying. 
     
     
         21 . The product of  claim 17 , wherein cross-linking includes contacting the tissue with a cross-linking agent. 
     
     
         22 . The product of  claim 21 , wherein the cross-linking agent includes at least one of glutaraldehyde, genepin, carbodiimides, and diisocyantes. 
     
     
         23 . The product of  claim 17 , wherein cross-linking includes heating the tissue. 
     
     
         24 . The product of  claim 23 , wherein the tissue is heated in a vacuum. 
     
     
         25 . The product of  claim 23 , wherein the tissue is heated to 70° C. to 120° C. 
     
     
         26 . The product of  claim 17 , further comprising placing the tissue in a mold to produce a desired shape prior to cross linking. 
     
     
         27 . The product of  claim 17 , wherein the tissue is cross-linked such that the material maintains the stable three-dimensional structure when contacted with an aqueous environment. 
     
     
         28 . The product of  claim 27 , wherein the material maintains the stable three-dimensional structure when implanted in a body. 
     
     
         29 . The product of  claim 17 , wherein the tissue contains hyaluronic acid and chondroitin sulfate. 
     
     
         30 . The product of  claim 17 , wherein the tissue contains one or more growth factors selected from PECAM-1, HGF, VEGF, PDGF-BB, follistatin, IL-8, and FGF-basic. 
     
     
         31 . The product of  claim 17 , wherein mechanically processing the tissue includes at least one of cutting, grinding, or blending to produce small particles. 
     
     
         32 . The product of  claim 17 , further comprising contacting the tissue with a flat surface and applying force to compress the tissue to a thickness of about 2.0 mm or less and optionally cross-linking the tissue. 
     
     
         33 . The product of  claim 17 , further comprising processing the tissue to form a drain or drain manifold in the form of a tube, column, or sheet, further processing the tissue to add grooves, channels, or holes, and cross-linking the tissue. 
     
     
         34 . The product of  claim 17 , further comprising processing the tissue to form thin strips have a thickness of up to about 2.0 mm, rolling the thin strips to form a drain in the shape of a hollow tube, and cross-linking the tissue. 
     
     
         35 - 55 . (canceled)