Pretreatment solution for immunohistochemical staining and condensed solution thereof

Abstract

Disclosed are a pretreatment solution for immunohistochemical staining, which elutes a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in the medium, and retrieves antigenicity of the tissue specimen, and which is usable three or more times, and a pretreatment solution concentrate for immunohistochemical staining which allows ready preparation of the pretreatment solution. The pretreatment solution for immunohistochemical staining contains an antigen retrieving agent, particular nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water. The content of the antigen retrieval agent is such that the pH of the pretreatment solution is in a predetermined range, and the content of cyclodextrin or a derivative thereof is a particular amount.

1 . A pretreatment solution for immunohistochemical staining for eluting a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in said medium, and for retrieving antigenicity of said tissue specimen, said pretreatment solution being usable three or more times,
 said pretreatment solution comprising an antigen retrieval agent, a polyoxyethylene alkyl phenyl ether nonionic surfactant, a polyoxyethylene sorbitan nonionic surfactant, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water, wherein a content of said antigen retrieval agent is such that a pH of said pretreatment solution is 5.0 to 10.0, and a content of said cyclodextrin or a derivative thereof is 0.01 to 1.0 mass %.   
     
     
         2 . The pretreatment solution for immunohistochemical staining according to  claim 1 , wherein a content of said polyoxyethylene alkyl phenyl ether nonionic surfactant is 0.01 to 1.0 mass %, and a content of said polyoxyethylene sorbitan nonionic surfactant is 0.3 to 3.0 mass %. 
     
     
         3 . The pretreatment solution for immunohistochemical staining according to  claim 1 , further comprising an amphiphilic solution. 
     
     
         4 . The pretreatment solution for immunohistochemical staining according to  claim 1 , wherein said antigen retrieval agent is a citrate buffer and/or a Tris buffer. 
     
     
         5 . The pretreatment solution for immunohistochemical staining according to  claim 1 , wherein said polyoxyethylene alkyl phenyl ether nonionic surfactant is at least one of NP-40, Triton X-100, Triton X-114, or IGEPAL CA-630, and said polyoxyethylene sorbitan nonionic surfactant is at least one of Tween 20, Tween 40, or Tween 80. 
     
     
         6 . The pretreatment solution for immunohistochemical staining according to  claim 1 , wherein said cyclodextrin or a derivative thereof is at least one of α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, hydroxyalkyl-α-cyclodextrin, hydroxyalkyl-β-cyclodextrin, and hydroxyalkyl-γ-cyclodextrin. 
     
     
         7 . The pretreatment solution for immunohistochemical staining according to  claim 2 , wherein said amphiphilic solution is at least one of ethylene glycol, propylene glycol, or 1,3-butylene glycol. 
     
     
         8 . The pretreatment solution for immunohistochemical staining according to  claim 1 , further comprising a preservative and/or a disinfectant. 
     
     
         9 . A pretreatment solution concentrate for immunohistochemical staining comprising less water than a pretreatment solution for immunohistochemical staining according to  claim 1 , wherein said pretreatment solution is for use as a pretreatment solution for immunohistochemical staining at a predetermined pH by adding a predetermined amount of water thereto upon use. 
     
     
         10 . The pretreatment solution concentrate for immunohistochemical staining according to  claim 9 , wherein said predetermined pH is 5.0 to 7.0, and said pretreatment solution concentrate is supplied in single part. 
     
     
         11 . The pretreatment solution concentrate for immunohistochemical staining according to  claim 9 , wherein said pretreatment solution concentrate is supplied in two parts and consists of a first solution concentrate comprising an antigen retrieval agent, and a second solution concentrate comprising a polyoxyethylene alkyl phenyl ether nonionic surfactant, polyoxyethylene sorbitan nonionic surfactant, and cyclodextrin or a derivative thereof, wherein said pretreatment solution concentrate is for use by mixing said first solution concentrate, said second solution concentrate, and a predetermined amount of water to set said predetermined pH to 8.0 to 10.0 
     
     
         12 . The pretreatment solution concentrate according to  claim 11 , wherein said second solution concentrate further comprising an amphiphilic solution.