Pre-Transplant Accommodated Organs Resistant To Anti-Donor Immunity

Abstract

This invention includes the composition of organ grafts accommodated prior to transplantation and therefore resistant to rejection by preformed antibodies. Accommodation is achieved within the donor animal by administration of sub-lethal levels of accommodation inducing factors derived from animals sensitized to the donor.

1 . A xenograft from a donor mammal prepared by a method comprising:
 (i) infusing a non-human pre-immune fetal donor mammal at least one time with sub-lethal levels of bone marrow,   (ii) allowing prolonged exposure of the donor mammal to the bone marrow,   (iii) harvesting a xenograft from the donor mammal; and   (iv) determining that the xenograft from the donor mammal is resistant to complement-dependent cytotoxicity mediated by preformed antibodies of a sensitized recipient mammal reactive with donor antigen,   
       wherein the xenograft is a tissue or an organ. 
     
     
         2 . The xenograft of  claim 1 , wherein the bone marrow is from a mammal which is of the same species as the sensitized recipient. 
     
     
         3 . The xenograft of  claim 1 , wherein the sensitized recipient mammal is a human. 
     
     
         4 . The xenograft of  claim 1 , wherein the bone marrow is from the sensitized recipient mammal. 
     
     
         5 . The xenograft of  claim 1 , wherein the xenograft is an organ and the organ is selected from the group consisting of heart, kidney, liver, lung, pancreas, intestine, spleen, and thymus. 
     
     
         6 . The xenograft of  claim 1 , wherein the xenograft is a tissue and the tissue is selected from the group consisting of bone, skin, neural tissue, skeletal muscle myocytes, smooth muscle myocytes, cardiac muscle myocytes, pancreatic islets, hepatocytes, stem cells, progenitor cells, and hematopoietic cells. 
     
     
         7 . The xenograft of  claim 1 , wherein the step of determining occurs prior to the step of harvesting.