Method and device for the collection of airborne particles and their concentration in small amounts of liquid

Abstract

A method and its possible embodiments are presented for collecting airborne biological and non-biological agents and concentrating them into small amounts of liquid. Airborne particles are drawn into the sampler and by the action of an electrostatic field are deposited on the collection electrode covered with superhydrophobic substance (surface contact angle >150°). At the end of the sampling period a small liquid droplet (5-60 μL) is injected at the top of the sampling chamber. Since the sampler is positioned at an angle to the horizontal, the droplet rolls-off of collection electrode's surface under the force of gravity and removes the deposited particles. The droplet is then collected by a liquid capture system. The particles concentrated in such small amounts of liquid facilitate sample analysis by various techniques. The suggested sampler's embodiments achieve sample concentration rates higher than 1×10 6 /min which will allow detection of very low particle concentrations.

1 . A method for collecting electrically charged airborne particles of biological and non-biological origin and concentrating them in small amounts of liquid, the method comprising:
 at least one air flow channel assembly comprising a first conductor and a second conductor, the first conductor disposed substantially parallel to the second conductor and wherein both said conductors are parallel to the direction of air flow through the channel assembly and whereby said air flow channel has an inlet and an outlet;   whereby one of the conductors of said assembly is connected to high voltage, while the other is grounded to effect creation of an electrostatic field in said air flow channel;   whereby one of said conductors is much narrower than the other conductor to effect the focusing of the electrostatic field toward the narrower conductor once the voltage is applied;   whereby the narrower conductor is covered with a superhydrophobic substance to ensure high contact angle for liquids placed on its surface;   whereby the entire flow channel is positioned at an angle to the horizontal;   whereby the electrically charged airborne particles pulled through said air flow channel are deposited onto the narrower conductor with the superhydrophobic surface under the action of the electrostatic field;   whereby a small liquid droplet placed near the air inlet of the air flow channel and on top of the conductor covered by a superhydrophobic coating rolls down under the force of gravity and picks up the particles that have been deposited onto said electrode with superhydrophobic coating;   whereby the droplet that has collected particles deposited on the conductor with the superhydrophobic coating is captured into a container positioned near the outlet of the air flow channel; and   whereby said droplet and particles contained wherein are analyzed by a variety of methods to determine the presence of biological and non-biological particles.   
     
     
         2 . The electrostatic air sampling device based on the  claim 1  comprising
 an electrostatic collection module comprising:
 an air flow channel assembly having a shape of a closed half cylinder, wherein one of the conductors have a shape of half cylinder; 
 the second conductor positioned in a plate enclosing the first conductor in the direction of air flow; and 
 whereby the second conductor is much narrower than the first conductor; and 
 whereby the second conductor is positioned opposite the apex of the first conductor; and 
 whereby the second conductor is slightly below the surface of a plane it is positioned in to form a groove; and 
 whereby the second conductor is covered with a superhydrophobic substance; and 
 whereby a voltage is applied across the two conductors, 
 
 a charger to electrically charge airborne particles, whereby said charger is positioned at the inlet of the electrostatic collection module to electrically charge the airborne particles being drawn into the module; and 
 an air mover for drawing airborne particles into the electrical charger, through the electrostatic collection module, and out through the outlet and whereby the air mover is positioned at the end of the electrostatic collection module; and 
 one or more power sources operatively connected to the particle charger, the electrostatic collection module, and to the air mover; and 
 a liquid pump, whereby said pump injects the droplets of various sizes and at various time intervals at the top of the air flow channel and into the groove containing the second conductor; and 
 a liquid capture system positioned between the electrostatic collection module and the air mover to capture the liquid droplet(s) injected at the top of the electrostatic collection module whereby said droplet(s) have accumulated particles that have been deposited onto the conductor coated with superhydrophobic substance. 
 
     
     
         3 . The electrostatic sampling device based on the  claim 1  where liquid droplets of volume less <100 μL are used to remove the particles deposited on the electrode covered with superhydrophobic substance. 
     
     
         4 . The electrostatic sampling device based on the  claim 1  where the width of the conductor with superhydrophobic coating can be of different sizes to accommodate various sizes of the collecting droplet. 
     
     
         5 . The electrostatic sampling device based on the  claim 1  where the conductor not covered with superhydrophobic substance is grounded and where the electrode that is covered with superhydrophobic substance is connected to high voltage and whereby said voltage has a sign opposite the sign of electrical charge of particles being sampled. 
     
     
         6 . The electrostatic sampling device based on the  claim 1  where the electrode with superhydrophobic coating is grounded and high voltage is applied to the other electrode and whereby said high voltage has the same sign as the sign of electrical charge of particles being sampled. 
     
     
         7 . The electrostatic sampling device based on the  claim 1  where the air flow channel is positioned at an angle to the horizontal with the angle being such as to ensure the roll-off of the droplets assisted only by the force of gravity. 
     
     
         8 . The electrostatic collection module based on the  claim 1  wherein the air flow channel assembly has a shape resembling that of an inverted triangle with apex facing downward; and
 one of the conductors have a shape of an arc with its highest point facing upward; and 
 the second conductor is positioned opposite the first conductor in the apex of the triangle; and 
 the second conductor is much narrower than the first conductor; and 
 the second conductor is slightly below the surface of a plane it is positioned in; and 
 the second conductor is covered with a superhydrophobic substance; and 
 the side walls of the triangle are at 60 degrees to horizontal; and 
 whereby most of the inside of said walls are covered by conducting inlays to minimize losses of charged particles. 
 
     
     
         9 . The electrostatic sampling device based on the  claim 2  where the collection liquid is aqueous buffer. 
     
     
         10 . The electrostatic sampling device based on the  claim 2  where the collection liquid is nutrient mixture.