US2012228228A1PendingUtilityA1

Methods of Preparation and Manufacture of Biocompatible Solid-Phase Microextraction Coatings and Coated Devices

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Individually held — no corporate assignee on recordPriority: Mar 11, 2002Filed: May 23, 2012Published: Sep 13, 2012
Est. expiryMar 11, 2022(expired)· nominal 20-yr term from priority
Inventors:Janusz B. PawliszynFlorin Marcel MusteataMihaela L. MusteataRobert E. ShireyLeonard M. SidiskyYong Chen
Y10T428/2933G01N 2030/062G01N 2030/009A61B 10/0045H01J 49/165B01J 20/283G01N 1/405A61B 5/150358
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Claims

Abstract

A process for manufacturing a fiber or other device with a biocompatible coating for using the fiber in solid phase microextraction (SPME) of a small molecule of interest from a matrix. The process includes the step of coating the fiber/device with a coating of a biocompatible polymer and a solvent having solid phase microextraction (SPME) particles with pores dimensioned to absorb the small molecule of interest from the matrix suspended therein. The process also includes the steps of drying the coated fiber/device to remove the solvent and curing the dried coated fiber at an elevated temperature.

Claims

exact text as granted — not AI-modified
1 . A process for coating a fiber with a biocompatible coating for use of the fiber in solid phase microextraction (SPME) of a small molecule of interest from a matrix, the process comprising the steps of:
 coating the fiber with a coating of a biocompatible polymer and a solvent having solid phase microextraction (SPME) particles with pores dimensioned to absorb the small molecule of interest from the matrix suspended therein;   drying the coated fiber to remove the solvent; and   curing the dried coated fiber at an elevated temperature.   
     
     
         2 . The process of  claim 1  wherein the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles, HS-F5/silica particles, normal-phase silica particles, C-1/silica particles, C-4/silica particles, C-6/silica particles, C-8/silica particles, C-30/silica particles, phenyl/silica particles, cyano/silica particles, ionic liquid/silica particles, molecular imprinted polymer particles, carboxen particles, divinylbenzene particles, diol/silica particles and mixtures thereof. 
     
     
         3 . The process of  claim 1  wherein the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles and HS-F5/silica particles. 
     
     
         4 . The process of  claims 1  wherein the biocompatible polymer is selected from the group consisting of polyacrylonitrile (PAN), polyethylene glycol, polypyrrole, derivatised cellulose, polysulfone and polyamide. 
     
     
         5 . The process of  claim 1  wherein the biocompatible polymer is polyacrylonitrile (PAN) or a co-polymer thereof. 
     
     
         6 . The process of  claim 1  wherein the solvent is selected from the group consisting of: dimethylformamide (DMF), dimethyl sulfoxide, NaSCN, Ca(CNS) 2 , nitric acid, ethylene carbonate and mixtures thereof. 
     
     
         7 . The process of  claim 1  wherein the solvent is dimethylformamide (DMF). 
     
     
         8 . The process of  claim 1  wherein the process of coating comprises the biocompatible polymer and the solvent being in a ratio of between about 5% and 15% biocompatible polymer/solvent (w/w). 
     
     
         9 . The process of  claim 8  wherein the biocompatible polymer/solvent ratio is between about 7.5% and 12% (w/w). 
     
     
         10 . The process of  claim 9  wherein the biocompatible polymer/solvent ratio is about 10% (w/w). 
     
     
         11 . The process of  claim 1  wherein a resulting SPME coating on the cured dried coated fiber comprises SPME particles and biocompatible polymer being in a ratio of about 0.3 and about 0.7 PAN/silica (w/w). 
     
     
         12 . The process of  claim 11  wherein the PAN/silica ratio is about 0.5 (w/w). 
     
     
         13 . The process of  claim 1  wherein the process of curing includes applying the elevated temperature at about 180° C. to about 210° C. and maintaining the fiber at the elevated temperature for about 5 seconds to about 1.5 minutes. 
     
     
         14 . The process of  claim 1  wherein the fiber is a metal wire is selected from the group consisting of stainless steel, titanium, a nickel-titanium alloy, and Nitinol. 
     
     
         15 . A process for preparing a device for use in solid phase microextraction (SPME) of a small molecule of interest from a biological matrix, the process comprising:
 identifying the small molecule of interest to be extracted from the matrix;   selecting extraction phase SPME particles having pores dimensioned to absorb the identified small molecule of interest from the matrix;   selecting a biocompatible polymer for suspending the SPME particles, the selection of the biocompatible polymer being responsive to the biocompatible polymers characteristic to reduce the adsorption of proteins and macromolecules onto the suspended SPME particles while allowing the SPME particles to extract the small molecule of interest from the matrix;   selecting a solvent for combining with the selected biocompatible polymer;   dissolving the selected biocompatible polymer in the selected solvent; and   combining the selected SPME particles with the combination of the dissolved biocompatible polymer and the solvent to form a solid phase microextraction (SPME) coating solution.   
     
     
         16 . The process of  claim 15 , further comprising:
 coating a fiber with the coating solution;   drying the coated fiber to remove the solvent; and   curing the dried coated fiber at an elevated temperature.   
     
     
         17 . The process of  claim 16 , further comprising:
 exposing the cured fiber to the biological matrix, the exposing including adsorbing a plurality of small molecules of interest by the pores of the suspended SPME particles of the dried coating on the cured fiber.   
     
     
         18 . The process of  claim 17  wherein the matrix is selected from the group consisting of biological fluid, tissues, organs, cells, whole blood, plasma, serum, urine, cerebrospinal fluid, saliva and peritoneal fluid. 
     
     
         19 . The process of  claim 16  wherein a SPME coating on the cured fiber comprises SPME particles and biocompatible polymer being in a ratio of about 0.3 and about 0.7 PAN/silica (w/w). 
     
     
         20 . The process of  claim 19  wherein the PAN/silica ratio is about 0.5 (w/w). 
     
     
         21 . The process of  claim 16  wherein the process of curing includes applying the elevated temperature at about 180° C. to about 210° C. and maintaining the fiber at the elevated temperature for about 5 seconds to about 1.5 minutes. 
     
     
         22 . The process of  claim 15  wherein the process of dissolving results in the biocompatible polymer and the solvent being in a ratio of between about 5% and 15% biocompatible polymer/solvent (w/w). 
     
     
         23 . The process of  claim 15  the process of dissolving results in the biocompatible polymer and the solvent being in a ratio of between about 7.5% and 12% (w/w). 
     
     
         24 . The process of  claim 15  the process of dissolving results in the biocompatible polymer and the solvent being in a ratio of about 10% (w/w). 
     
     
         25 . The process of  claim 15  wherein the process of selecting the extraction phase SPME particles includes selecting the SPME particles as a function of a SPME characteristic selected from the group consisting of: a) SPME particles having a size of about 1.7 μm to about 50 μm particles; b) SPME particles having a pore size from about 10 Å to about 200 Å; c) SPME particles have a pore size from about 80 Å to about 180 Å; and d) SPME particles having a surface area of about 200 m2/g to about 800 m2/g. 
     
     
         26 . The process of  claim 15  wherein the process of selecting the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles, HS-F5/silica particles, normal-phase silica particles, C-1/silica particles, C-4/silica particles, C-6/silica particles, C-8/silica particles, C-30/silica particles, phenyl/silica particles, cyano/silica particles, ionic liquid/silica particles, molecular imprinted polymer particles, carboxen particles, divinylbenzene particles, diol/silica particles and mixtures thereof. 
     
     
         27 . The process of  claim 15  wherein the process of selecting the biocompatible polymer including selecting a biocompatible polymer from the group consisting of polyacrylonitrile (PAN), a co-polymer of polyacrylonitrile (PAN), polyethylene glycol, polypyrrole, derivatised cellulose, polysulfone and polyamide. 
     
     
         28 . The process of  claim 15  wherein the process of selecting a solvent includes selecting a solvent from the group consisting of dimethylformamide (DMF), dimethyl sulfoxide, NaSCN, Ca(CNS) 2 , nitric acid, ethylene carbonate and mixtures thereof.

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