Human platelet f11 receptor

Abstract

The present invention is directed to isolated nucleic acid molecules encoding human platelet F11 receptors. Expression vectors and host cells comprising the nucleic acid molecules are also provided, as well as methods for increasing or decreasing the expression of the human platelet F11 receptor in host cells. The invention further provides a method of screening a substance for the ability of the substance to modify human platelet F11 receptor function, and a method for isolating other human platelet F11 receptor molecules. DNA oligomers capable of hybridizing to the nucleic acid molecule encoding the human platelet F11 receptor are provided, which can be used to detect human platelet F11 receptor in a sample.

1 . A DNA oligomer capable of hybridizing in full-length under high stringency conditions to the full length of a nucleic acid molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, nucleotides 16-912 of SEQ ID NO:1 and nucleotides 97-912 of SEQ ID NO: 1, wherein the high stringency hybridization conditions are overnight hybridization at about 68° C. in 6×SSC and a wash in 6×SSC at room temperature, followed with a wash at 68° C. first in 6×SSC and then in 0.6×SSC, wherein the DNA oligomer has a length of 1822 nucleotides when the DNA oligomer hybridizes to SEQ ID NO: 1, the DNA oligomer has a length of 897 nucleotides when the DNA oligomer hybridizes to nucleotides 16-912 of SEQ ID NO:1 and the DNA oligomer has a length of 816 nucleotides when the DNA oligomer hybridizes to nucleotides 97-912 of SEQ ID NO: 1. 
     
     
         2 . A DNA oligomer consisting of a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, nucleotides 16-912 of SEQ ID NO:1 and nucleotides 97-912 of SEQ ID NO: 1, or a nucleotide sequence complementary thereto. 
     
     
         3 . A DNA oligomer capable of hybridizing in full-length under high stringency conditions to the full length of a nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, nucleotides 16-912 of SEQ ID NO:1 and nucleotides 97-912 of SEQ ID NO: 1, wherein the high stringency hybridization conditions are overnight hybridization at about 68° C. in 6×SSC and a wash in 6×SSC at room temperature, followed with a wash at 68° C. first in 6×SSC and then in 0.6×SSC, wherein the DNA oligomer has a length of 1822 nucleotides when the DNA oligomer hybridizes to SEQ ID NO: 1, the DNA oligomer has a length of 897 nucleotides when the DNA oligomer hybridizes to nucleotides 16-912 of SEQ ID NO:1 and the DNA oligomer has a length of 816 nucleotides when the DNA oligomer hybridizes to nucleotides 97-912 of SEQ ID NO: 1. 
     
     
         4 . The DNA oligomer of  claim 1 , wherein the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, and nucleotides 97-912 of SEQ ID NO: 1 encodes an amino acid sequence selected from the group consisting of SEQ ID NO:3 and amino acid residues 28-299 of SEQ ID NO:3. 
     
     
         5 . The DNA oligomer of  claim 2 , wherein the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, and nucleotides 97-912 of SEQ ID NO: 1 encodes an amino acid sequence selected from the group consisting of SEQ ID NO:3 and amino acid residues 28-299 of SEQ ID NO:3. 
     
     
         6 . The DNA oligomer of  claim 3 , wherein the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, and nucleotides 97-912 of SEQ ID NO: 1 encodes an amino acid sequence selected from the group consisting of SEQ ID NO:3 and amino acid residues 28-299 of SEQ ID NO:3.