Compositions and Methods for Implantation of Processed Adipose Tissue and Processed Adipose Tissue Products

Abstract

The invention provides compositions and methods for the preparation of processed adipose tissue. The invention further provides methods of use of the processed adipose tissue.

1 . A composition comprising processed adipose tissue comprising a decellularized adipose tissue extracellular matrix to which viable cells securely attach. 
     
     
         2 . The composition of  claim 1 , wherein the composition further comprises at least one material from the group consisting of anesthetic, analgesic, antibiotic, antimicrobial, growth factors, cryopreservative, antioxidant, free radical scavenger, caspase inhibitor, vitamin, lipoaspirate, and a cell. 
     
     
         3 . The composition of  claim 1 , further comprising a cross-linking agent. 
     
     
         4 . The composition of  claim 3 , wherein the cross-linking agent is selected from the group consisting of carbodiimide (EDC), hexamethylene diisocyanate (HMDC), gluteraldehyde, proanthocyanidin, ribose, threose, and lysyl oxidase, carbodiimide, polyepoxy ethers, divinyl sulfone (DVS), genipin, polyaldehyde and diphenylphosphoryl azide (DPPA), genipin, epoxy compounds, dialdehyde starch, glutaraldehyde, formaldehyde, dimethyl suberimidate, carbodiimides, succinimidyls, diisocyanates, and acyl azide; or any combination thereof. 
     
     
         5 . The composition of  claim 1 , further comprising a biopolymer scaffold. 
     
     
         6 . The composition of  claim 5 , wherein the processed adipose tissue and biopolymer scaffold further comprise a biopolymer cross-linking agent. 
     
     
         7 .- 8 . (canceled) 
     
     
         9 . The composition of claim  8 , wherein the mixture further comprises a polymerization agent. 
     
     
         10 . (canceled) 
     
     
         11 .- 12 . (canceled) 
     
     
         13 . The composition of  claim 1 , wherein the processed adipose tissue is essentially free of a basement membrane or wherein the processed adipose tissue includes a basement membrane. 
     
     
         14 . The composition of  claim 1 , wherein the composition is substantially non-immunogenic when implanted in a subject. 
     
     
         15 .- 25 . (canceled) 
     
     
         26 . A method for preparation of processed adipose tissue (PAT) comprising sequentially:
 a) providing mammalian tissue comprising solid adipose;   b) isolating the adipose from the non-adipose material in the tissue; and   c) decellularizing the adipose.   
     
     
         27 .- 29 . (canceled) 
     
     
         30 . The method of  claim 26 , wherein decellularizing the adipose or extracting lipid from the adipose comprises contacting the adipose with a weak acid and a non-ionic detergent. 
     
     
         31 .- 39 . (canceled) 
     
     
         40 . The method of  claim 30 , further comprising combining the processed adipose tissue and biopolymer scaffold with a biopolymer cross-linking agent. 
     
     
         41 .- 44 . (canceled) 
     
     
         45 . The method of  claim 40 , wherein the biocompatible polymer comprises a polymer selected from the group consisting of hyaluronic acid, PEG-DA, chondroitin sulfate, partially or fully hydrolyzed poly(vinyl alcohol), poly(vinylpyrrolidone), poly(ethyloxazoline), poly(ethylene oxide)-co-poly(propylene oxide) block copolymers (poloxamers and meroxapols), poloxamines, carboxymethyl cellulose, and hydroxyalkylated celluloses such as hydroxyethyl cellulose and methylhydroxypropyl cellulose, polypeptides, polysaccharides or carbohydrates, Ficoll® polysucrose, dextran, heparan sulfate, heparin, alginate, gelatin, collagen, albumin, ovalbumin, and copolymers or blends thereof. 
     
     
         46 .- 47 . (canceled) 
     
     
         48 . The method of  claim 45 , further comprising contacting the adipose with a nuclease. 
     
     
         49 . The method of  claim 48 , wherein the adipose is contacted with the nuclease after step (c). 
     
     
         50 . (canceled) 
     
     
         51 . The method of  claim 45 , further comprising contacting the adipose with at least one material from the group consisting of anesthetic, antibiotic, growth factors, cryopreservative, antioxidant, free radical scavenger, caspase inhibitor, vitamin, lipoaspirate, and a cell. 
     
     
         52 . The method of  claim 51 , wherein the adipose is contacted with the at least on material after step (c). 
     
     
         53 . The method of any of  claim 52 , wherein the adipose tissue of step (c) is essentially free of a basement membrane. 
     
     
         54 .- 60 . (canceled) 
     
     
         61 . A method of implanting processed adipose tissue in a subject comprising:
 a) identifying a subject in need of implantation of processed adipose tissue;   b) identifying a site in the subject in need of implantation of processed adipose tissue; and   c) implanting the processed adipose tissue in the subject at the identified site.   
     
     
         62 . The method of  claim 61 , wherein the processed tissue is made by the process comprising:
 a) providing mammalian tissue comprising solid adipose;   b) isolating the adipose from the non-adipose material in the tissue; and   c) decellularizing the adipose.   
     
     
         63 . (canceled)