US2012167257A1PendingUtilityA1

Method for altering the metabolism of plant

49
Individually held — no corporate assignee on recordPriority: Apr 7, 1999Filed: Mar 6, 2012Published: Jun 28, 2012
Est. expiryApr 7, 2019(expired)· nominal 20-yr term from priority
Inventors:Mark A. HollandJoseph C. Polacco
C12R 2001/01C12N 1/205C12N 1/20C12N 5/04C12N 15/01A01N 63/20
49
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Claims

Abstract

The invention is related to a method of altering the metabolism of a plant by carrying out the steps of treating seeds of the plant with non-genetically engineered selected mutant pink-pigmented facultative methylotroph having a plant altering capability, and growing the plants from the treated seeds, wherein the plants that grow from such treated seeds exhibit an altered characteristic relative to non-treated plants.

Claims

exact text as granted — not AI-modified
1 - 19 . (canceled) 
     
     
         20 . A method of increasing vitamin B12 content of a plant or tissue thereof, comprising growing a plant from plant tissue treated with a mutant pink-pigmented facultative methylotroph (PPFM) selected for overproduction of vitamin B12, wherein the plant or a tissue thereof that is grown from the treated plant tissue exhibits an increase in vitamin B12 content relative to a non-treated plant or tissue thereof. 
     
     
         21 . The method according to  claim 20 , wherein the mutant pink-pigmented facultative methylotroph produces at least 7.5 ng vitamin B12 per ml of culture supernatant. 
     
     
         22 . The method according to  claim 20 , wherein the mutant pink-pigmented facultative methylotroph produces at least 15 ng vitamin B12 per ml of culture supernatant. 
     
     
         23 . The method according to  claim 20 , wherein said mutant is  Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium extorquens, Methylobacterium fujisawaense, Methylobacterium radiotolerans, Methylobacterium rhodesianum, Methylobacterium rhodinum , or  Methylobacterium zatmanii.    
     
     
         24 . The method according to  claim 20 , wherein said plant or tissue thereof exhibits a vitamin B12 content of at least about 0.01 micrograms per gram fresh weight of tissue. 
     
     
         25 . The method according to  claim 20 , wherein said plant or tissue thereof exhibits a vitamin B12 content of at least about 0.1 micrograms per gram fresh weight of tissue. 
     
     
         26 . The method according to  claim 23 , wherein said mutant is  Methylobacterium mesophilicum.    
     
     
         27 . The method according to  claim 20 , wherein said plant is a soybean plant or a lettuce plant. 
     
     
         28 . The method according to  claim 20 , wherein said tissue is a seed or a leaf 
     
     
         29 . The method of  claim 28 , wherein said seed is heat-treated before being treated with mutant pink-pigmented facultative methylotroph (PPFM). 
     
     
         30 . A method for selecting a mutant pink-pigmented facultative methylotroph that overproduces vitamin B12 for treatment of plants, comprising selecting a pink-pigmented facultative methylotroph for stimulation of vitamin B12 auxotroph growth, wherein said selected pink-pigmented facultative methylotroph provides for an increase in vitamin B12 content in a treated plant or tissue relative to a non-treated plant or tissue thereof. 
     
     
         31 . The method of  claim 30 , wherein said methylotroph produces at least 7.5 ng vitamin B12 per ml of culture supernatant. 
     
     
         32 . The method according to  claim 30 , wherein said methylotroph produces at least 15 ng vitamin B12 per ml of culture supernatant. 
     
     
         33 . The method of  claim 30 , wherein said mutant is  Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium extorquens, Methylobacterium fujisawaense, Methylobacterium radiotolerans, Methylobacterium rhodesianum, Methylobacterium rhodinum  or  Methylobacterium zatmanii.    
     
     
         34 . The method of  claim 30 , wherein said treated plant or tissue thereof exhibits a vitamin B12 content of at least about 0.01 micrograms per gram fresh weight of tissue. 
     
     
         35 . The method according to  claim 30 , wherein said treated plant or tissue thereof exhibits a vitamin B12 content of at least about 0.1 micrograms per gram fresh weight of tissue. 
     
     
         36 . The method of  claim 30 , wherein said mutant was isolated from a natural sample or was subjected to a mutagenizing amount of chemicals, irradiation, or stress. 
     
     
         37 . A plant tissue comprising a pink-pigmented facultative methylotroph mutant selected for overproduction of vitamin B12. 
     
     
         38 . The isolated plant tissue according to  claim 37 , wherein said methylotroph produces at least 7.5 ng vitamin B12 per ml of culture supernatant. 
     
     
         39 . The isolated plant tissue according to  claim 37 , wherein said methylotroph produces at least 15 ng vitamin B12 per ml of culture supernatant. 
     
     
         40 . The plant tissue according to  claim 37 , wherein said mutant is  Methylobacterium mesophilicum, Methylobacterium organophilum, Methylobacterium extorquens, Methylobacterium fujisawaense, Methylobacterium radiotolerans, Methylobacterium rhodesianum, Methylobacterium rhodinum , or  Methylobacterium zatmanii.    
     
     
         41 . The plant tissue according to  claim 37 , wherein said plant tissue exhibits a vitamin B12 content of at least about 0.01 micrograms per gram fresh weight of tissue. 
     
     
         42 . The method according to  claim 37 , wherein said treated plant tissue exhibits a vitamin B12 content of at least about 0.1 micrograms per gram fresh weight of tissue. 
     
     
         43 . The plant tissue according to  claim 37 , wherein said tissue is a seed or a leaf 
     
     
         44 . The plant tissue according to  claim 37 , wherein said plant is a soybean plant or lettuce.

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