Yeast Strain and Method for Using the Same to Produce Nicotinamide Riboside

Abstract

The present invention embraces a fungal strain deficient in nicotinamide riboside import and salvage and use thereof for producing nicotinamide riboside. Methods for producing nicotinamide riboside and a nicotinamide riboside-supplemented food product using the strain of the invention are also provided.

1 . An isolated fungal strain deficient in nicotinamide riboside import and salvage. 
     
     
         2 . The fungal strain of  claim 1 , wherein said strain does not express Nicotinamide Riboside Kinase 1 (Nrk1), Uridine Hydrolase 1 (Urh1), Purine Nucleoside Phosphorylase (Pnp1), and Nicotinamide Riboside Transporter 1 (Nrt1). 
     
     
         3 . The fungal strain of  claim 1 , wherein said strain secretes at least 8 mg/L nicotinamide riboside. 
     
     
         4 . The fungal strain of  claim 1 , wherein said fungus is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Kluveromyres, Aspergillus  and  Pichia.    
     
     
         5 . The fungal strain of  claim 1 , wherein said fungus is  Saccharomyces cerevisiae.    
     
     
         6 . A method for producing nicotinamide riboside comprising culturing the fungal strain of  claim 1  in culture medium and recovering nicotinamide riboside from the medium thereby producing nicotinamide riboside. 
     
     
         7 . The method of  claim 6 , wherein the culture medium comprises nicotinic acid or nicotinamide. 
     
     
         8 . The method of  claim 6 , wherein the fungal strain is cultured to an optical density of at least 3. 
     
     
         9 . The method of  claim 6 , wherein the nicotinamide riboside is recovered by solubilizing nicotinamide riboside from the medium with methanol and subjecting the nicotinamide riboside to column chromatography. 
     
     
         10 . A method for producing a nicotinamide riboside-supplemented food product comprising providing a fermentable substrate and fermenting the fermentable substrate in the presence of the fungal strain of  claim 1  thereby producing a nicotinamide riboside supplemented food product. 
     
     
         11 . A nicotinamide riboside supplemented food product fermented in the presence of the fungal strain of  claim 1 . 
     
     
         12 . The product of  claim 11 , wherein said food product is wine, beer, cider, kvass, root beer, soy sauce or bread.