US2012152828A1PendingUtilityA1

Inhibition of biofilm formation and removal of biofilm by use of moss

Individually held — no corporate assignee on recordPriority: Aug 30, 2010Filed: Aug 30, 2011Published: Jun 21, 2012
Est. expiryAug 30, 2030(~4.1 yrs left)· nominal 20-yr term from priority
A01N 65/00C02F 3/32
58
PatentIndex Score
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Claims

Abstract

A method of inhibiting biofilm growth on a surface in an aqueous system comprising contacting a surface susceptible to biofilm growth with a solution comprising an amount of a non-decomposed moss or non-decomposed moss extract effective to inhibit biofilm growth. A method of removing biofilm from a surface in an aqueous system comprising contacting a surface having a biofilm with a solution containing an amount of a non-decomposed moss or non-decomposed moss extract effective to remove some or all of the biofilm from the surface.

Claims

exact text as granted — not AI-modified
1 . A method of inhibiting biofilm growth on a surface in an aqueous system comprising contacting a surface susceptible to biofilm growth with a solution comprising an amount of a non-decomposed moss or non-decomposed moss extract effective to inhibit biofilm growth. 
     
     
         2 . The method of  claim 1 , wherein the solution comprises a non-decomposed moss. 
     
     
         3 . The method of  claim 2 , wherein the non-decomposed moss is in the form of leaves or parts of leaves. 
     
     
         4 . The method of  claim 3 , wherein the non-decomposed moss is in the form of compressed leaves or parts of leaves. 
     
     
         5 . The method of  claim 2 , wherein the non-decomposed moss is placed in a carrier. 
     
     
         6 . The method of  claim 5 , wherein the carrier is a mesh bag. 
     
     
         7 . The method of  claim 1 , wherein the solution comprises a non-decomposed moss extract. 
     
     
         8 . The method of  claim 1 , wherein the aqueous system is a spa, swimming pool, aquarium, splash deck, water tower, holding tank, pond, cooling tower, water bottle, or toilet. 
     
     
         9 . The method of  claim 1 , wherein the solution is prepared and then contacted with the surface. 
     
     
         10 . The method of  claim 2 , wherein the solution is prepared in situ by placing non-decomposed moss in the aqueous system. 
     
     
         11 . The method of  claim 1 , wherein the amount of non-decomposed moss or non-decomposed moss extract is effective to inhibit biofilm growth by 30 percent or more after 48 hours. 
     
     
         12 . The method of  claim 1 , wherein the amount of non-decomposed moss or non-decomposed moss extract is effective to inhibit biofilm growth by 50 percent or more after 48 hours. 
     
     
         13 . The method of  claim 1 , wherein the amount of non-decomposed moss or non-decomposed moss extract is effective to inhibit biofilm growth by 70 percent or more after 48 hours. 
     
     
         14 . The method of  claim 12 , wherein the percent inhibition is measured by crystal violet staining. 
     
     
         15 . The method of  claim 1 , wherein the moss or moss extract is selected from the group consisting of sphagnum papillosum, sphagnum cristatum, and mixtures thereof. 
     
     
         16 . A method of removing biofilm from a surface in an aqueous system comprising contacting a surface having a biofilm with a solution containing an amount of a non-decomposed moss or non-decomposed moss extract effective to remove some or all of the biofilm from the surface. 
     
     
         17 . The method of  claim 16 , wherein the solution comprises a non-decomposed moss. 
     
     
         18 . The method of  claim 17 , wherein the non-decomposed moss is in the form of leaves or parts of leaves. 
     
     
         19 . The method of  claim 18 , wherein the non-decomposed moss is in the form of compressed leaves or parts of leaves. 
     
     
         20 . The method of  claim 17 , wherein the non-decomposed moss is placed in a carrier. 
     
     
         21 . The method of  claim 20 , wherein the carrier is a mesh bag. 
     
     
         22 . The method of  claim 16 , wherein the solution comprises a non-decomposed moss extract. 
     
     
         23 . The method of  claim 16 , wherein the aqueous system is a spa, swimming pool, aquarium, splash deck, water tower, holding tank, pond, cooling tower, water bottle, or toilet. 
     
     
         24 . The method of  claim 16 , wherein the solution is prepared and then contacted with the surface. 
     
     
         25 . The method of  claim 17 , wherein the solution is prepared in situ by placing non-decomposed moss in the aqueous system. 
     
     
         26 . The method of  claim 16 , wherein the amount of non-decomposed moss or non-decomposed moss extract is effective to remove biofilm by 30 percent or more after 48 hours. 
     
     
         27 . The method of  claim 16 , wherein the amount of non-decomposed moss or non-decomposed moss extract is effective to remove biofilm by 50 percent or more after 48 hours. 
     
     
         28 . The method of  claim 16 , wherein the amount of non-decomposed moss or non-decomposed moss extract is effective to remove biofilm by 70 percent or more after 48 hours. 
     
     
         29 . The method of  claim 26 , wherein the percent removal is measured by crystal violet staining. 
     
     
         30 . The method of  claim 16 , wherein the moss or moss extract is selected from the group consisting of sphagnum papillosum, sphagnum cristatum, and mixtures thereof.

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