Method for electroeluting genetic material from dried samples

Abstract

In accordance with the present disclosure, a method for extracting genetic material from a biological sample stored on a solid medium is provided. The method includes obtaining the solid medium, wherein the biological sample is applied on the solid medium, and the solid medium includes chemicals that lysed the biological sample and preserved the genetic material. The method also includes electroeluting the genetic material directly from the solid medium to a subsequent medium.

1 . A method for extracting genetic material from a biological sample stored on a solid medium, comprising:
 obtaining the solid medium, wherein the biological sample is applied on the solid medium, and the solid medium comprises chemicals that lyse the biological sample and preserve the genetic material; and   electroeluting the genetic material directly from the solid medium to a subsequent medium.   
     
     
         2 . The method of  claim 1 , wherein the solid medium comprises a cellulose-based material. 
     
     
         3 . The method of  claim 1 , comprising rinsing the solid medium prior to electroelution. 
     
     
         4 . The method of  claim 3 , wherein rinsing the solid medium comprises soaking the solid medium in buffer or flowing buffer through or across the solid medium. 
     
     
         5 . The method of  claim 3 , wherein rinsing the solid medium comprises soaking the solid medium in water or flowing water through or across the solid medium. 
     
     
         6 . The method of  claim 3 , further comprising repairing the genetic material prior to electroelution. 
     
     
         7 . The method of  claim 6 , wherein repairing the genetic material comprises applying a solution containing at least DNA polymerase and DNA ligase. 
     
     
         8 . The method of  claim 7 , wherein the DNA polymerase comprises DNA polymerase activity, 3′ to 5′ exonuclease activity for proofreading, and 5′ to 3′ exonuclease activity for nick translation. 
     
     
         9 . The method of  claim 6 , wherein repairing the genetic material comprises applying a solution containing at least an endonuclease that nicks DNA adjacent to abasic sites. 
     
     
         10 . The method of  claim 1 , wherein the biological sample comprises fixed cells. 
     
     
         11 . The method of  claim 10 , comprising processing the fixed cells prior to electroelution from the solid medium, wherein processing comprises rehydrating the fixed cells, treating the fixed cells with protease, or reversing cross-linking. 
     
     
         12 . The method of  claim 1 , wherein the subsequent medium comprises an electrophoresis gel, a solution, or a capture surface. 
     
     
         13 . A method for extracting genetic material from a fixed tissue sample stored on a solid medium, comprising:
 applying at least a portion of the fixed tissue sample to the solid medium, wherein the solid medium comprises chemicals that can lyse the tissue sample or preserve the genetic material; and   electroeluting the genetic material directly from the solid medium to a subsequent medium.   
     
     
         14 . The method of  claim 13 , wherein the solid medium comprises a cellulose-based paper. 
     
     
         15 . The method of  claim 13 , wherein the solid medium comprises FTA paper. 
     
     
         16 . The method of  claim 13 , comprising treating the fixed tissue sample with protease prior to or after applying the fixed tissue sample to the solid medium. 
     
     
         17 . The method of  claim 13 , wherein the solid medium comprises chemicals that lyse the fixed tissue sample. 
     
     
         18 . The method of  claim 13 , comprising rehydrating the fixed tissue sample prior to applying the fixed tissue sample to the solid medium. 
     
     
         19 . The method of  claim 18 , comprising reversing cross-linking between the genetic material and protein prior to or subsequent to applying the fixed tissue sample to the solid medium. 
     
     
         20 . The method of  claim 19 , wherein reversing cross-linking comprises applying crosslink repair buffer comprising a nucleophile with a pH other than 7.0. 
     
     
         21 . The method of  claim 14 , comprising repairing the genetic material prior to electroelution. 
     
     
         22 . The method of  claim 21 , wherein repairing the genetic material comprises applying a solution containing at least DNA polymerase I and DNA ligase. 
     
     
         23 . The method of  claim 21 , wherein repairing the genetic material comprises applying a solution containing at least an endonuclease that nicks DNA adjacent to abasic sites. 
     
     
         24 . The method of  claim 13 , wherein the subsequent medium comprises an electrophoresis gel, a solution, or a capture surface. 
     
     
         25 . A method for extracting DNA from a biological sample stored on a cellulose-based paper, comprising:
 obtaining the cellulose-based paper, wherein the biological sample is applied on the cellulose-based paper, and the cellulose-based paper comprises chemicals that lyse the biological sample and preserve the DNA;   repairing the DNA on the cellulose-based paper; and   electroeluting DNA directly from the cellulose-based paper into an electrophoresis gel.