Method for Producing Glucosamine by Culturing Microorganism with Low-cost Medium

Abstract

A method for producing glucosamine with microorganism comprises of fermenting with a microorganism selected from the group consisting of Monascus pilosus and Aspergillus sp. in a novel low-cost medium, thereby enable it to produce glucosamine; wherein said medium is consisted of commercial Taiwan sugar, soy beam, rice bran and the like; wherein suitable condition for the fermentation is: 150˜300 rpm, pH 4˜pH 8, and 24° C.˜37° C.; wherein, after fermentation culturing, the fermentation liquor is filtered with suction to recover said microorganism biomass, said microorganism biomass is then subjected to steps of cell disruption, hydrochloric acid reaction, neutralization reaction and filtration, to obtain glucosamine produced by the microorganism.

1 . A method for producing glucosamine with a microorganism, comprising fermenting with  Monascus pilosus , comprising fermenting a liquid medium with a  Monascus pilosus  under conditions suitable to produce glucosamine, wherein the liquid medium comprises 25 g/L rice bran, 25 g/L white crystal sugar and 15 g/L NH4Cl. 
     
     
         2 . A method for producing glucosamine with a microorganism as recited in  claim 1 , wherein the microorganism is fermented under condition of pH 4˜pH 6. 
     
     
         3 . A method for producing glucosamine with a microorganism as recited in  claim 1 , wherein, after fermentation of the microorganism, the fermentation liquor is filtered with suction to recover the microorganism biomass, and then the microorganism biomass is subjected to steps of cell disruption with a cell disrupter, hydrochloric acid reaction, neutralization reaction and filtration to obtain glucosamine produced by the microorganism. 
     
     
         4 . A method for producing glucosamine with microorganism as recited in  claim 3 , wherein the hydrochloric acid reaction is carried out with 1 g wet biomass and 6 N HCl at 100° C. for 4 hours to retain a glucosamine concentration approaching a stationary value. 
     
     
         5 . A method for producing glucosamine with microorganism as recited in  claim 3 , wherein the neutralization reaction is carried out with NaOH to neutralize the reaction solution to pH 7.