Novel paenibacillus polymyxa strain, antibiotic antimicrobial agents and methods of their use

Abstract

The present invention relates to a novel Paenibacillus polymyxa strain, OSY-DF, and its bioactive mutants. Also provided is a method for using a novel antimicrobial peptide, paenibacillin, isolated from the bacterial strain OSY-DF, and its bioactive variants or fragments. The invention also relates to antimicrobial compositions containing same and methods of their use.

1 - 44 . (canceled) 
     
     
         45 . An isolated  Paenibacillus polymyxa  strain OSY-DF, registered under accession no. ATCC PTA-7852. 
     
     
         46 . A composition comprising a  Paenibacillus polymyxa  strain OSY-DF, registered under accession no. ATCC PTA-7852, a fermentate of a live culture of the OSY-DF, or a crude extract of a live culture of the OSY-DF. 
     
     
         47 . The composition of  claim 46 , wherein said composition comprises the  Paenibacillus polymyxa  strain OSY-DF, registered under accession no. ATCC PTA-7852. 
     
     
         48 . The composition of  claim 46 , wherein said composition comprises the fermentate of a live culture of the  Paenibacillus polymyxa  strain OSY-DF. 
     
     
         49 . The composition of  claim 46 , wherein said composition comprises the crude extract of a live culture of the  Paenibacillus polymyxa  strain OSY-DF. 
     
     
         50 . The composition of  claim 46 , wherein said composition is a pharmaceutical preparation suitable for treatment of bacterial infection in a subject, and wherein said composition further comprises a pharmaceutically acceptable carrier. 
     
     
         51 . The composition of  claim 46 , wherein said composition is a pharmaceutical preparation suitable for prevention of bacterial colonization in a subject, and wherein said composition further comprises a pharmaceutically acceptable carrier. 
     
     
         52 . The composition of  claim 46 , wherein said composition is a probiotic preparation suitable for oral administration to a subject. 
     
     
         53 . The composition of  claim 46 , wherein said composition is suitable for use in controlling bacterial colonization of an environment. 
     
     
         54 . The composition of  claim 53 , wherein said environment is a food product. 
     
     
         55 . The composition of  claim 53 , wherein said environment is a non-biological surface. 
     
     
         56 . The composition of  claim 46 , wherein said composition is suitable for use as a biocontrol agent for the control of plant pathogenic bacteria. 
     
     
         57 . A method of preventing or treating a bacterial infection in a subject, comprising administering to said subject an effective amount of a composition comprising a  Paenibacillus polymyxa  strain OSY-DF, registered under accession no. ATCC PTA-7852, a fermentate of a live culture of the OSY-DF, or a crude extract of a live culture of the OSY-DF. 
     
     
         58 . The method of  claim 57 , wherein the bacterial infection is caused by one or more Gram-positive bacteria selected from  Bacillus  spp.,  Clostridium sporogenes, Lactobacillus  spp.,  Lactococcus lactis, Leuconostoc mesenteroides, Listeria  spp.,  Pediococcus cerevisiae, Staphylococcus aureus, Streptococcus agalactiae , or combinations thereof. 
     
     
         59 . The method of  claim 57 , wherein the bacterial infection is caused by one or more Gram-negative bacteria selected from  Acinetobacter  spp.,  Escherichia coli, Pseudomonas  spp.,  Salmonella enterica serovars, Yersinia enterocolitica , or combinations thereof. 
     
     
         60 . The method of  claim 57 , wherein the method of preventing or treating is prophylactic. 
     
     
         61 . A method for preventing or inhibiting the growth of bacteria on or inside an environment comprising introducing onto one or more surfaces of the environment an effective amount of a composition comprising a  Paenibacillus polymyxa  strain OSY-DF, registered under accession no ATCC PTA-7852, a fermentate of a live culture of the OSY-DF, or a crude extract of a live culture of the OSY-DF. 
     
     
         62 . The method of  claim 61 , wherein the environment is selected from an organism, a food product, an agricultural crop, and a non-biological surface. 
     
     
         63 . The method of  claim 61 , wherein the bacteria whose growth is to be prevented or inhibited comprise one or more Gram-positive bacteria selected from  Bacillus  spp.,  Clostridium  sporogenes,  Lactobacillus  spp.,  Lactococcus lactis, Leuconostoc mesenteroides, Listeria  spp.,  Pediococcus cerevisiae, Staphylococcus aureus, Streptococcus agalactiae , or combinations thereof. 
     
     
         64 . The method of  claim 61 , wherein the bacteria whose growth is to be prevented or inhibited comprise one or more Gram-negative bacteria selected from  Acinetobacter  spp.,  Escherichia coli, Pseudomonas  spp.,  Salmonella enterica serovars, Yersinia enterocolitica , or combinations thereof.