Marker and reagent for detection of human il-17-producing helper t cells, and method for detection of human il-17-producing helper t cells

Abstract

The present invention relates to a marker allowing specific detection of human IL-17-producing helper T-cells (human Th17 cells), a method for specifically detecting human Th17 cells and a reagent for detecting human Th17 cells.

1 . A polynucleotide marker for detecting human IL-17-producing helper T-cells which is a polynucleotide having a nucleic acid sequence of at least one gene selected from the group consisting of:
 genes encoding membrane proteins consisting of: ADAM12, ANKS1B, ATP6V0A4, ATP9A, BVES, C5orf40, CDH4, DIO2, DMD, GPR34, IRS2, KCNE3, L1CAM, MCAM, MFAP3L, MYO7A, PTPRM, SHROOM2, SLC16A4, SLCO2B1, TANC2, TJP1, TMEM163, TNS3, UPK1B, WDFY3, DRD2, GJC1, PGBD5 (LOC100134440), MS4A7, ODZ4, PHKA1, RGS1, SHB, SLC44A3, SLC6A15, SYNGR3, AKAP12, C9orf125, DPY19L2, HRH4, MUC20, POPDC3, SORBS1, TANC1, TMEM44 and UNC13C;   genes encoding secretory proteins consisting of: CXCL13, PCOLCE2, PNOC, SMPDL3A, TGFBI, C17orf99, EBI3, IL1A and WNT3;   genes encoding intracellular proteins consisting of: BCAT1, BHLHE22, C13orf18 (LOC728970), CA2, CCDC3, CDS1, CHN1, CLIC5 (LOC100131610), CTSH, CYP7B1, DAPK2, DMRT1, DSE, FBXL17, FBXL21, FHOD3, H2AFY2, HLX, IRAK3, MACC1, MAML3, MYO10, OTUB2, PAPSS2, PCBP3, PDE4DIP, PLD1, PPARG, PTPN13, RGS18, SIM1, SNAI2, SOX2, SPIRE1, TBC1D12, TGM5, TMOD1, TUBB6, DDIT4L, DHRS9, ERC2, FERMT2, HHEX, HS3ST1, NR5A2, PHLDA1, RBM20, NINL, RTN2, SH3RF2, TSHZ2, EML1, HIST1H2BC, MAP3K4, PDK4, RGS2 and RGS20;   genes consisting of: C1orf106, C6orf145, LOC401097, MAMLD1, ZC3H12C, C12orf64, C6orf168, CAMSAP1L1 and MAGED4 (MAGED4B); and   genes comprising at least one nucleic acid sequence selected from SEQ ID NO: 147 to 151, 157 to 162 and 167 to 174;   or a variant and fragment thereof.   
     
     
         2 . The marker according to  claim 1 , which is a polynucleotide having a nucleic acid sequence of at least one gene selected from the group consisting of:
 genes encoding membrane proteins consisting of: ADAM12, ATP6V0A4, ATP9A, BVES, C5orf40, CDH4, DIO2, GPR34, L1CAM, MCAM, PTPRM, SHROOM2, TMEM163, UPK1B, DRD2, PGBD5 (LOC100134440), ODZ4, SLC6A15, AKAP12, C9orf125, POPDC3 and UNC13C;   genes encoding secretory proteins consisting of: PCOLCE2, PNOC, TGFBI and IL1A; and   genes encoding intracellular proteins consisting of BHLHE22, PPARG, SIM1 and SNAI2.   
     
     
         3 . A protein marker for detecting human IL-17-producing helper T-cells which is a protein encoded by at least one gene according to  claim 1  or a functionally equivalent variant and fragment thereof. 
     
     
         4 . The marker according to  claim 3 , which is a protein encoded by at least one gene selected from the group consisting of:
 genes encoding membrane proteins consisting of: ADAM12, ATP6V0A4, ATP9A, BVES, C5orf40, CDH4, DIO2, GPR34, L1CAM, MCAM, PTPRM, SHROOM2, TMEM163, UPK1B, DRD2, PGBD5 (LOC100134440), ODZ4, SLC6A15, AKAP12, C9orf125, POPDC3 and UNC13C;   genes encoding secretory proteins consisting of: PCOLCE2, PNOC, TGFBI and IL1A; and   genes encoding intracellular proteins consisting of: BHLHE22, PPARG, SIM1 and SNAI2.   
     
     
         5 . The marker according to  claim 4 , wherein the protein is a membrane protein encoded by at least one gene selected from the group consisting of GPR34, MCAM and PTPRM. 
     
     
         6 . A method for detecting human IL-17-producing helper T-cells comprising detecting the presence of at least one polynucleotide marker for detecting human Th17 cells according to  claim 1  or at least one protein marker which is a protein encoded by at least one gene according to  claim 1  or a functionally equivalent variant and fragment thereof in a sample containing cells derived from human. 
     
     
         7 . The method according to  claim 6 , wherein the polynucleotide marker is detected with a nucleic acid probe that specifically hybridizes to the polynucleotide marker. 
     
     
         8 . The method according to  claim 7 , wherein the nucleic acid probe is a primer set for amplifying the polynucleotide marker by a nucleic acid amplification method. 
     
     
         9 . The method according to  claim 6 , wherein the protein marker is detected with a nucleic acid aptamer, antibody, ligand or receptor that specifically binds to the protein marker. 
     
     
         10 . A reagent for detecting human IL-17-producing helper T-cells comprising at least one substance selected from
 a nucleic acid probe that specifically hybridizes to the polynucleotide marker according to  claim 1 ; and   a nucleic acid aptamer, antibody, ligand or receptor that specifically binds to a protein marker which is a protein encoded by at least one gene according to  claim 1  or a functionally equivalent variant and fragment thereof.