US2012122686A1PendingUtilityA1

Novel glyphosate-n-acetyltransferase (gat) genes

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Individually held — no corporate assignee on recordPriority: Oct 30, 2000Filed: Nov 18, 2011Published: May 17, 2012
Est. expiryOct 30, 2020(expired)· nominal 20-yr term from priority
Inventors:Linda A. CastleDan SiehlLorraine GiverJeremy MinshullChristina IvyYong ChenPhillip A. PattenRebecca GortonNicholas B. DuckBilly F. MccutchenRoger Kemble
C12N 9/1029C12N 15/8209C12N 15/8275C12Y 205/01019C12N 9/1092
65
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Claims

Abstract

Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Claims

exact text as granted — not AI-modified
1 . A transgenic plant cell comprising a heterologous polypeptide with glyphosate N-acetyltransferase activity, wherein said plant cell produces N-acetylglyphosate when treated with glyphosate. 
     
     
         2 . The transgenic plant cell of  claim 1 , wherein said plant cell exhibits enhanced tolerance to glyphosate as compared to a wild-type plant cell of the same species, strain or cultivar. 
     
     
         3 . The transgenic plant cell of  claim 1 , further comprising at least one polypeptide imparting glyphosate tolerance by an additional mechanism. 
     
     
         4 . The transgenic plant cell of  claim 3 , wherein the at least one polypeptide imparting glyphosate tolerance by an additional mechanism is a glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase or a glyphosate oxido-reductase. 
     
     
         5 . The transgenic plant cell of  claim 1 , further comprising at least one polypeptide imparting tolerance to an additional herbicide. 
     
     
         6 . The transgenic plant cell of  claim 5 , wherein the at least one polypeptide imparting tolerance to an additional herbicide is a mutated hydroxyphenylpyruvatedioxygenase, a sulfonamide-tolerant acetolactate synthase, a sulfonamide-tolerant acetohydroxy acid synthase, an imidazolinone-tolerant acetolactate synthase, an imidazolinone-tolerant acetohydroxy acid synthase, a phosphinothricin acetyl transferase or a mutated protoporphyrinogen oxidase. 
     
     
         7 . The transgenic plant cell of  claim 5 , wherein the at least one polypeptide imparting tolerance to an additional herbicide comprises the highly resistant ALS (HRA) mutation of acetolactate synthase. 
     
     
         8 . The transgenic plant cell of  claim 1 , wherein the transgenic plant cell is from a crop plant selected from among the genera:  Eleusine, Lollium, Bambusa, Brassica, Dactylis, Sorghum, Pennisetum, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Coix, Glycine  and  Gossypium.    
     
     
         9 . The transgenic plant cell of  claim 1 , wherein said polypeptide having glyphosate N-acetyltransferase activity has a k cat /K m  of at least 100 mM −1  min −1  for glyphosate. 
     
     
         10 . A transgenic seed comprising the plant cell of  claim 1 . 
     
     
         11 . A transgenic plant explant comprising the plant cell of  claim 1 . 
     
     
         12 . A plant cell comprising a metabolic product of glyphosate which is N-acetylglyphosate. 
     
     
         13 . The plant cell of  claim 12 , wherein the plant cell is from a crop plant selected from among the genera:  Eleusine, Lollium, Bambusa, Brassica, Dactylis, Sorghum, Pennisetum, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Coix, Glycine  or  Gossypium.    
     
     
         14 . A plant comprising the plant cell of  claim 12 . 
     
     
         15 . The plant of  claim 14 , wherein the plant is from a crop plant selected from among the genera:  Eleusine, Lollium, Bambusa, Brassica, Dactylis, Sorghum, Pennisetum, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Coix, Glycine  or  Gossypium.    
     
     
         16 . A method for selectively controlling weeds in a field containing a crop comprising:
 (a) planting a field with crop seeds or plants comprising a heterologous polypeptide with glyphosate N-acetyltransferase activity; and   (b) applying to the crop and weeds in the field a sufficient amount of glyphosate to control the weeds without significantly affecting the crop.   
     
     
         17 . The method of  claim 16 , wherein said crop seed or plants are selected from the group of genera consisting of:  Eleusine, Lollium, Bambusa, Brassica, Dactylis, Sorghum, Pennisetum, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Coix, Glycine  or  Gossypium.    
     
     
         18 . The method of  claim 16 , further comprising applying to the crop and weeds in the field a simultaneous or chronologically staggered application of glyphosate and an additional herbicide. 
     
     
         19 . The method of  claim 18 , wherein the additional herbicide is applied and comprises a hydroxyphenylpyruvatedioxygenase inhibitor, sulfonamide, imidazolinone, bialaphos, phosphinothricin, azafenidin, butafenacil, sulfosate, glufosinate, or a protox inhibitor. 
     
     
         20 . The method of  claim 16 , wherein said crop seeds or plants further comprise at least one gene encoding a polypeptide imparting glyphosate tolerance by an additional mechanism. 
     
     
         21 . The method of  claim 16 , wherein said crop seeds or plants further comprise at least one polypeptide imparting tolerance to an additional herbicide; and, applying to the crop and weeds in the field a simultaneous or chronologically staggered application of glyphosate and the additional herbicide which is sufficient to inhibit the growth of the weeds in the field without significantly affecting the crop. 
     
     
         22 . The method of  claim 21 , wherein said polypeptide imparting tolerance to the additional herbicide comprises a mutated hydroxyphenylpyruvatedioxygenase, a sulfonamide-tolerant acetolactate synthase, a sulfonamide-tolerant acetohydroxy acid synthase, an imidazolinone-tolerant acetolactate synthase, a phosphinothricin acetyl transferase or a mutated protoporphyrinogen oxidase. 
     
     
         23 . The method of  claim 21 , wherein at least one polypeptide imparting tolerance to an additional herbicide inhibits the activity of acetolactate synthase. 
     
     
         24 . The method of  claim 20 , wherein the at least one polypeptide imparting glyphosate tolerance by an additional mechanism is glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase or a glyphosate oxido-reductase. 
     
     
         25 . The method of  claim 16 , wherein said polypeptide having glyphosate N-acetyltransferase activity has a k cat /K m  of at least 100 mM −1  min −1  for glyphosate. 
     
     
         26 . A method for controlling weeds in a field containing a crop or seed comprising:
 (a) growing a plant that exhibits glyphosate tolerance, wherein the glyphosate tolerance is due to the in vivo expression of a polypeptide that binds to and detoxifies glyphosate without producing a primary glyphosate metabolite in the plant; and   (b) applying an effective amount of glyphosate to the crop and weeds in the field to control the weeds without significantly affecting the crop.   
     
     
         27 . The method of  claim 26 , wherein the primary metabolite of glyphosate is aminomethylphosphonic acid (AMPA). 
     
     
         28 . The method of  claim 26 , wherein said crop plant is selected from the group of genera consisting of:  Eleusine, Lollium, Bambusa, Brassica, Dactylis, Sorghum, Pennisetum, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Coix, Glycine  or  Gossypium.    
     
     
         29 . The method of  claim 26 , wherein said polypeptide having glyphosate N-acetyltransferase activity has a k cat /K m  of at least 100 mM −1  min −1  for glyphosate. 
     
     
         30 . The method of  claim 26 , wherein said crop plant further comprise at least one gene encoding a polypeptide imparting glyphosate tolerance by an additional mechanism. 
     
     
         31 . The method of  claim 30 , wherein the at least one polypeptide imparting glyphosate tolerance by an additional mechanism is glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase or glyphosate-tolerant glyphosate oxido-reductase. 
     
     
         32 . The method of  claim 26 , wherein said crop plant further comprise at least one polypeptide imparting tolerance to an additional herbicide. 
     
     
         33 . The method of  claim 32 , wherein said polypeptide imparting tolerance to the additional herbicide comprises a mutated hydroxyphenylpyruvatedioxygenase, a sulfonamide-tolerant acetolactate synthase, a sulfonamide-tolerant acetohydroxy acid synthase, an imidazolinone-tolerant acetolactate synthase, a phosphinothricin acetyl transferase or a mutated protoporphyrinogen oxidase. 
     
     
         34 . The method of  claim 32 , wherein at least one polypeptide imparting tolerance to an additional herbicide inhibits the activity of acetolactate synthase. 
     
     
         35 . The method of  claim 32 , wherein the at least one polypeptide imparting tolerance to an additional herbicide comprises the highly resistant ALS (HRA) mutation of acetolactate synthase.

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